Protein Info for PGA1_c20940 in Phaeobacter inhibens DSM 17395

Annotation: PHA depolymerase PhaZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 85% identity to sit:TM1040_1716)

Predicted SEED Role

"Polyhydroxyalkanoate depolymerase, intracellular"

KEGG Metabolic Maps

Butanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E248 at UniProt or InterPro

Protein Sequence (424 amino acids)

>PGA1_c20940 PHA depolymerase PhaZ (Phaeobacter inhibens DSM 17395)
MRYMMSYDLMETMRNTNQWLGATALSMASNPAFAAIPNPALSWMAAWGEVTERSYQRMVT
KPDWGIYTQTCADGRDHVVEIDTVVERPFGDLIHFRVAGRAEQPRKVLLVAPMSGHYATL
LRSTVKSLIENCEVYVTDWHNARDIPVSEGKFDIEDYTLYLVDFMREMGPDTHVIAVCQP
APLTLAATAYLAEQDPEAQPCSLTLIGGPVDPDATPTDVTDFGRRVTMGQLEETMIQRVG
FKYKGVGRKVYPGLLQLSSFMSMNGERHSKAFMDQIQRVSRGEASDHDAHNRFYDEYLAV
MDMTAEFYLSTVERVFKGGEIARNEFVVAGHKVDIGKITDVAVKTVEGANDDISAPGQCI
AALDLCTGLPETKKASHVEPGAGHYGIFAGRSWRDNIRPLVIEFMNANARKTKKPAAKRA
KATA