Protein Info for Psest_2094 in Pseudomonas stutzeri RCH2

Annotation: Predicted Fe-S-cluster redox enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 346 PF04055: Radical_SAM" amino acids 103 to 259 (157 residues), 43.7 bits, see alignment E=1.7e-15

Best Hits

Swiss-Prot: 95% identical to Y2231_PSEU5: Probable RNA methyltransferase PST_2231 (PST_2231) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 95% identity to psa:PST_2231)

Predicted SEED Role

"Probable RNA methyltransferase PA1839"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIR9 at UniProt or InterPro

Protein Sequence (346 amino acids)

>Psest_2094 Predicted Fe-S-cluster redox enzyme (Pseudomonas stutzeri RCH2)
MRIPDLQQRLADIGAKPRHIGRMTRAWLKGLPLDVGRRQQQAEDFLPLSVREGLPALNAE
IDGLARLRSEHPAADGSARLLVELADGQMVESVLLPRDGLCVSSQVGCAVGCVFCMTGKS
GLLRQLGSAEIVAQVALARRFRPVKKVVFMGMGEPAHNLDNVLEAIDLLGTDGGIGHKNL
VFSTVGDMRVFERLPQQRVKPALALSLHSTDAALRQALLPRAPQIAPDELVDLGEAYARA
TGFPIQYQWTLLKGINDNQDEMDGILRLLKGKYAVMNLIPYNSLEDDEYQRPDGERIVQI
VRYLHSRGVLTKVRNSAGQDIDGGCGQLRARAEQALDRKRRVDRGA