Protein Info for Psest_2085 in Pseudomonas stutzeri RCH2

Annotation: Periplasmic serine proteases (ClpP class)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 transmembrane" amino acids 7 to 29 (23 residues), see Phobius details amino acids 179 to 196 (18 residues), see Phobius details PF08496: Peptidase_S49_N" amino acids 2 to 147 (146 residues), 189.4 bits, see alignment E=3.7e-60 PF01343: Peptidase_S49" amino acids 151 to 299 (149 residues), 149.5 bits, see alignment E=7.9e-48

Best Hits

Swiss-Prot: 52% identical to SOHB_SHIFL: Probable protease SohB (sohB) from Shigella flexneri

KEGG orthology group: K04774, serine protease SohB [EC: 3.4.21.-] (inferred from 98% identity to psa:PST_2240)

Predicted SEED Role

"Peptidase, U7 family"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMR9 at UniProt or InterPro

Protein Sequence (340 amino acids)

>Psest_2085 Periplasmic serine proteases (ClpP class) (Pseudomonas stutzeri RCH2)
MEFLVDYASFLAKTVTLVVAIVIVLIALASMRRGRSKQGGGHLEVHKLNEFYKSMRERLE
QAVLEKDAFKALRKREAKAAKQNKKPETPARVFVLDFDGDIRASAVDNLRHEITALLTMA
TPQDEVVLRLESGGGMVHGYGLAASQLVRIRDAGVPLTVCVDKVAASGGYMMACIGNRIL
TAPFAILGSIGVVAQLPNVHRLLKKHDVDFEVLTAGEYKRTLTVFGENTEKGREKFQEDL
ETTHELFKNFVARYRQNLSIDDVATGEVWLGIAALGKHLADELKTSDQYLAERAQEAELF
QLHYIQKKSLPERFGMAASTVMEHGLLKGWSRLNEQRFWQ