Protein Info for PGA1_c20540 in Phaeobacter inhibens DSM 17395

Annotation: ATP-dependent protease La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 PF02190: LON_substr_bdg" amino acids 9 to 199 (191 residues), 187.1 bits, see alignment E=1.3e-58 TIGR00763: endopeptidase La" amino acids 10 to 768 (759 residues), 993.4 bits, see alignment E=5e-303 PF07728: AAA_5" amino acids 350 to 430 (81 residues), 29.4 bits, see alignment E=2.5e-10 PF00004: AAA" amino acids 350 to 486 (137 residues), 84.4 bits, see alignment E=3.4e-27 PF22667: Lon_lid" amino acids 510 to 563 (54 residues), 80.1 bits, see alignment 2.7e-26 PF05362: Lon_C" amino acids 567 to 769 (203 residues), 311.1 bits, see alignment E=1.1e-96 PF13541: ChlI" amino acids 617 to 737 (121 residues), 30.1 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 69% identical to LON_RHIME: Lon protease (lon) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 90% identity to sit:TM1040_0775)

MetaCyc: 60% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E1W9 at UniProt or InterPro

Protein Sequence (804 amino acids)

>PGA1_c20540 ATP-dependent protease La (Phaeobacter inhibens DSM 17395)
MQEPLNSSYPVLPLRDIVVFPHMIVPLFVGREKSVRALEEVMTDDKQILLSSQIDPAEDD
PQSDGIYNVGVLANVLQLLKLPDGTVKVLVEGHARVKITEYLENDNFFEARAEYLTEMPG
DVTTVEALLRTVGDEFERYAKVRKNIPEEALSAVGETTEPAKLADLVAGHLGIEVDNKQD
LLETLSISERLEKVYGLMQGELSVLQVEKKIKTRVKTQMEKTQREYYLNEQMKAIQKELG
DSEDGSNEVAELEAKIAETKLSKEAREKSEAELKKLKNMSPMSAEATVVRNYLDWILALP
WGTKSRVKKDLSRAQDILDADHYGLEKVKERIVEYLAVQQRSAKLKGPILCLVGPPGVGK
TSLGKSVAKATGREFIRISLGGVRDESEIRGHRRTYIGSMPGKIIQALKKAKTTNPLILL
DEIDKMGQDFRGDPASAMLEVLDPEQNATFMDHYLEVEYDLSNVMFLTTSNSYNMPGPLL
DRMEIIPLSGYTEDEKREIAKQHLISKQVKNHGLKAKEFELTDEALTEIIRTYTREAGVR
NLEREIAKVARKSLTKIVKKEAETVSVTGDNLDDFLGVAKYRYGLAEKEDQVGVVTGLAY
TSVGGELLSIEALRLPGKGRMKTTGKLGDVMKESIEAASSYVRSISPQLGVKPPKFDKID
IHVHVPDGATPKDGPSAGLAMVTAIVSVLTGIPVRKDIAMTGEVTLRGNATAIGGLKEKL
LAALRGGIKTVLIPQENEKDLPDIPDNVKEGLTIIPVSHVSEVLKHALTDTPEPIEWDEA
AEEAAAAAKAALGAPDDAAVATAH