Protein Info for Psest_2060 in Pseudomonas stutzeri RCH2

Annotation: PQQ-dependent dehydrogenase, methanol/ethanol family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 28 to 557 (530 residues), 804.3 bits, see alignment E=2.5e-246 PF13360: PQQ_2" amino acids 72 to 237 (166 residues), 64.3 bits, see alignment E=2e-21 amino acids 471 to 539 (69 residues), 37.7 bits, see alignment E=2.8e-13 PF01011: PQQ" amino acids 95 to 126 (32 residues), 30 bits, see alignment (E = 4.4e-11) amino acids 145 to 171 (27 residues), 21.1 bits, see alignment (E = 3e-08) amino acids 503 to 539 (37 residues), 28.4 bits, see alignment 1.5e-10 PF13570: PQQ_3" amino acids 114 to 162 (49 residues), 20.7 bits, see alignment 6.7e-08 amino acids 481 to 521 (41 residues), 29.2 bits, see alignment 1.4e-10

Best Hits

Swiss-Prot: 53% identical to EXAA_PSEAE: Quinoprotein alcohol dehydrogenase (cytochrome c) (exaA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 95% identity to psa:PST_2265)

MetaCyc: 53% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"quinoprotein alcohol dehydrogenase" in subsystem Respiratory dehydrogenases 1

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8

Use Curated BLAST to search for 1.1.2.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMP2 at UniProt or InterPro

Protein Sequence (592 amino acids)

>Psest_2060 PQQ-dependent dehydrogenase, methanol/ethanol family (Pseudomonas stutzeri RCH2)
MKHTGLRKPFALTALCAAVAMSSLHAWAVTDQEILNDAKSTDQIVTNGMGLQGQRYSTLD
ALNANNINQLRPVWGFSLGGEKQRGQEAQPLIKDGVMYITGSYSRVYAVDARTGKELWQY
DARLPDGIMPCCDVINRGVALYDDLVIFGTLDAKLVALNKDTGKVVWKKTVADYKAGYSL
TAAPLIVKGKLITGVSGGEFGVVGKIEAYNPKNGELLWTRPTVEGHMGYVWKDGKKVESG
ISGGEAGKTWPGDLWKTGGAAPWLGGYYDPDTDSLLFGTGNPAPWNSHLRPGDNLYSSSR
LALDPNDGSIKWHFQTTPHDGWDYDGVNELVSFDYQEGGKTIKAAGTADRNGFFYVLDRT
NGKFIRGFPFVDKITWAKGLDKNGRPIYDDANRPGSPNDAGAQGKTVTVAPSFLGGKNWM
PMAYSQDTGLFYVPSNEWSMDIWNEGTAYKKGAAYLGAGFTIHPLNDDYIGVLRAIDPKT
GKEVWRYKNYAPLWGGVLATKGNLVFTGNPEGYLMAFDAKTGKKLYEFNTGSGVIGSPVT
WEMDGEQYVSVLSGWGGAVPLWGGEVAKRVKEFNQGGMVWTFKLPKDQVAKR