Protein Info for Psest_2054 in Pseudomonas stutzeri RCH2

Annotation: PQQ-dependent dehydrogenase, methanol/ethanol family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 34 to 586 (553 residues), 778 bits, see alignment E=2.3e-238 PF13360: PQQ_2" amino acids 81 to 238 (158 residues), 57.8 bits, see alignment E=2e-19 amino acids 500 to 568 (69 residues), 30.3 bits, see alignment E=4.9e-11 PF01011: PQQ" amino acids 103 to 128 (26 residues), 25.4 bits, see alignment (E = 1.3e-09) amino acids 152 to 177 (26 residues), 22.1 bits, see alignment (E = 1.4e-08) amino acids 532 to 568 (37 residues), 39.5 bits, see alignment 4.4e-14 PF13570: PQQ_3" amino acids 122 to 170 (49 residues), 22 bits, see alignment 2.8e-08 amino acids 510 to 550 (41 residues), 20.2 bits, see alignment 9.8e-08

Best Hits

Swiss-Prot: 86% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 96% identity to psa:PST_2270)

MetaCyc: 84% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"quinoprotein alcohol dehydrogenase" in subsystem Respiratory dehydrogenases 1

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIN2 at UniProt or InterPro

Protein Sequence (623 amino acids)

>Psest_2054 PQQ-dependent dehydrogenase, methanol/ethanol family (Pseudomonas stutzeri RCH2)
MTTRSNPGSKRPLAIAVHYLALAGSLALAGAAHAKPVSWEDIANDHLSTANVLQYGMGTN
AQRWSPLAQVNESNVFKLTPAWSFSFGDEKQRGQESQAIVHDGVIYVTGSYSRVFALDAK
TGKRLWSYAHRLPDDIRPCCDVVNRGAAIYGDKIYFGTLDARVVALNKDTGKVVWNKKFG
DHAAGYTMTGAPTLVKDGKTGKVLLVHGSSGDEFGIVGKLFARDPETGEEVWMRPFVEGH
MGRLNGKDSTPTGDIKAPSWPDDPNHPTGKKEAWSQGGGAPWQSASFDPETNTIIVGAGN
PAPWNGWERTSDGGNPKDYDSLYTSGQVGVDPSTGEVKWFYQHTPNDAWDFSGNNELVLF
DYKDNNGKTVKATAHADRNGFFYVVDRKDGKLQNAFPFVDGITWASHIDLKTGRPVENQG
QRPPKPEPGEKHGKSVEVSPPFLGGKNWNPMAYSQDTGLFYVPANHWKEDYWTEEVSYKK
GSAYLGQGFRIKRMYDDHVGILRAMNPTTGKIEWEHKEKLPLWAGVLATKGNLIFTGTSD
GYFKAFNAKTGDELWKFQTGSGVISPPVTWEQDGEQYIGVTVGYGGAVPLWGGDMAVLTK
PVAQGGSFWVFKLPEWEKKTAKR