Protein Info for Psest_0202 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 186 to 208 (23 residues), see Phobius details amino acids 215 to 238 (24 residues), see Phobius details amino acids 250 to 270 (21 residues), see Phobius details amino acids 278 to 300 (23 residues), see Phobius details amino acids 306 to 328 (23 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 367 to 386 (20 residues), see Phobius details PF07696: 7TMR-DISMED2" amino acids 54 to 172 (119 residues), 67.8 bits, see alignment E=1.5e-22 PF07695: 7TMR-DISM_7TM" amino acids 188 to 388 (201 residues), 136.4 bits, see alignment E=2e-43 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 461 to 625 (165 residues), 171.5 bits, see alignment E=6.1e-55 PF00990: GGDEF" amino acids 465 to 622 (158 residues), 162.9 bits, see alignment E=8.5e-52

Best Hits

KEGG orthology group: K13590, diguanylate cyclase (inferred from 83% identity to psa:PST_4044)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GDI6 at UniProt or InterPro

Protein Sequence (643 amino acids)

>Psest_0202 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MIRTFLLLLACLTTLPWIASVSAAPQTSVLQTRTEAALQVGVVEYLLGEPAADFVDIAAA
DLQWQALTKPNLGKQRDGAWLRFTLYNEGAEAKRWYLLLKWPVLDRVAVRLHYPDSDRWG
QPMLAGDALALSVRPLADHHFVYPLELPAGEPVVVYMQLQAREILALPLELIDDKQLIEG
KLRDVTLVSLFFGGILVIVLYNCSLLIFTRDRSYFLYVLYLLSAVFYVLTITGFGQLYLW
PEIPELSARFYGLSAALCFFTPMLFALRFLGIRRYGGWVWAVSITLTCYWGLVALTILLA
PTLARYLFMESVALLHCVLTMAVTLNLWARGNQSARLFSIAWSTLLGFTVINLLALNGTL
PLNAWTLNGQLIGMFTEFVLLSMALAERINVERNRRIAAQQLALQASETLAEERALHLQA
KQEALDLQLHTNEVLEARVYERTRALEEVRQGLEAANAELTRLSTTDALTQLANRRRFDR
LLDEEIRRARRSGSPLSVLLADIDHFKRVNDGYGHPFGDECLRQVAAVLAAHCQRAGDLA
ARYGGEEFVVLLPASDQRQAFVLAERIRQNIERLQLRYGDQAVALTISLGVATLGPTVAS
AEALLATADAALYAAKHAGRNQVVCAPEAAIGETGAGVSPQPV