Protein Info for Psest_0020 in Pseudomonas stutzeri RCH2

Annotation: K+ transport systems, NAD-binding component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF02254: TrkA_N" amino acids 3 to 104 (102 residues), 88.7 bits, see alignment E=1.7e-28 amino acids 234 to 349 (116 residues), 88.9 bits, see alignment E=1.4e-28 PF02080: TrkA_C" amino acids 154 to 223 (70 residues), 46.5 bits, see alignment E=1.3e-15 amino acids 390 to 450 (61 residues), 37.7 bits, see alignment E=6.8e-13

Best Hits

Swiss-Prot: 74% identical to TRKA_HALED: Trk system potassium uptake protein TrkA (trkA) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K03499, trk system potassium uptake protein TrkA (inferred from 99% identity to psa:PST_0018)

Predicted SEED Role

"Trk system potassium uptake protein TrkA" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Conserved gene cluster associated with Met-tRNA formyltransferase or Glutathione-regulated potassium-efflux system and associated functions or Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GD41 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Psest_0020 K+ transport systems, NAD-binding component (Pseudomonas stutzeri RCH2)
MKIIILGAGQVGGTLAEHLASEANDITVVDTDGERLRDLGDRLDIRTIQGRGSFPTVLRQ
AGADDADMLVAVTNSDETNMVACQVAYTLFHTPTKIARVRESAYLVHSGLFNNEAIPVDV
LISPEQVVTNYIKRLIEHPGSLQVIDFAGGKAQLVAVRAYYGGPLVGQELRQLRRHMPNV
DTRVAAIFRRNHAILPQGDTVIEADDEVFFIAAKGHIRAVMSEMRRLDENYKRIVIAGGG
HIGERLAEAIESRYQVKIIEMNPARCRYLSDTLDSTIILQGSASDRDLLVEENINEADVF
LALTNDDEANIMSSLLAKRLGARKVMCIINNPAYVDLVQGGEIDIAISPQLATIGTLLTH
VRRGDIVSAHSLRRGAAEAIEVIAHGDPRSSKVVGRAIEQIALPPGTTIGAVIRDDQVLI
AHDDTRIESGDHVILFLVDKKHIRDVERLFQAGLTFF