Protein Info for PGA1_c00020 in Phaeobacter inhibens DSM 17395

Annotation: DNA polymerase III subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 PF00712: DNA_pol3_beta" amino acids 1 to 120 (120 residues), 108.8 bits, see alignment E=2.9e-35 TIGR00663: DNA polymerase III, beta subunit" amino acids 1 to 372 (372 residues), 310.2 bits, see alignment E=8.9e-97 PF02767: DNA_pol3_beta_2" amino acids 134 to 250 (117 residues), 104.4 bits, see alignment E=7.1e-34 PF02768: DNA_pol3_beta_3" amino acids 253 to 372 (120 residues), 111.9 bits, see alignment E=2.5e-36

Best Hits

Swiss-Prot: 52% identical to DPO3B_CAUVC: Beta sliding clamp (dnaN) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K02338, DNA polymerase III subunit beta [EC: 2.7.7.7] (inferred from 95% identity to sit:TM1040_0002)

Predicted SEED Role

"DNA polymerase III beta subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DKX3 at UniProt or InterPro

Protein Sequence (373 amino acids)

>PGA1_c00020 DNA polymerase III subunit beta (Phaeobacter inhibens DSM 17395)
MKISIERGTLLKAVAQAQSVVERRNTIPILANVLIEAEGDIVQFRATDLDIEVVDKAPAQ
VERAGATTVAATTLHEIVRKLPDGALVTLTADAASGRLTVEAGRSNFSLATLPREDFPVM
ASSEYHSNFSANAAVLQRLFDKSKFAISTEETRYYLNGVYMHVADGVEGGKALRCVATDG
HRLARIDANLPMGAEDMPGVIVPRKTVGELRKLLDDDEMDIAVSVSETKVRFATPNITLT
SKVIDGTFPDYTRVIPAGNTRRLEVDASEFARAVDRVATVSSERSRAVKLQLDEDRLILS
VNAPDSGAAEEELAVAYRDERLEIGFNAKYLLEIANQVDRENAVFMFNSSGDPTLMREGS
DESAVYVVMPMRV