Protein Info for HP15_1932 in Marinobacter adhaerens HP15

Annotation: methylisocitrate lyase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 TIGR02317: methylisocitrate lyase" amino acids 8 to 291 (284 residues), 428.9 bits, see alignment E=4e-133 PF13714: PEP_mutase" amino acids 11 to 254 (244 residues), 163.9 bits, see alignment E=5.1e-52 PF00463: ICL" amino acids 74 to 172 (99 residues), 39.4 bits, see alignment E=2.9e-14

Best Hits

Swiss-Prot: 70% identical to PRPB_VIBCH: 2-methylisocitrate lyase (prpB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03417, methylisocitrate lyase [EC: 4.1.3.30] (inferred from 93% identity to maq:Maqu_1666)

MetaCyc: 66% identical to 2-methylcitrate lyase (Cupriavidus necator)
Methylisocitrate lyase. [EC: 4.1.3.30]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PQ16 at UniProt or InterPro

Protein Sequence (295 amino acids)

>HP15_1932 methylisocitrate lyase 2 (Marinobacter adhaerens HP15)
MSNKLSPGARFRKALKDNQPLQIVGTINAYAAMMAEKVGHQAIYLSGGGVANASYGLPDL
GMTSMNEVVEDVRRITAATDVPLLVDIDTGWGGAFNIARTIREMERAGAAAVHIEDQVAQ
KRCGHRPNKEIVSQEEMVDRIKAAVDAREDKDFFIMARTDAFQKEGLEAAIERAKACIEA
GADGIFAEAVTELEHYKAFSEALDVPILANITEFGATPLYNRKELAEAGADMVLYPLSAF
RAMNKAALTVYQNILEKGDQKDVVDMMQTRMELYDFLNYHDFEQKLDQLFQQSKG