Protein Info for HP15_1930 in Marinobacter adhaerens HP15

Annotation: aconitate hydratase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 860 (859 residues), 1755.7 bits, E=0
• TIGR01341: aconitate hydratase 1
  • amino acids 20 to 857 (838 residues), 960.4 bits, E=7.1e-293
• PF00330: Aconitase
  • amino acids 64 to 533 (470 residues), 462.2 bits, E=2.2e-142
• PF00694: Aconitase_C
  • amino acids 657 to 789 (133 residues), 97.9 bits, E=5.9e-32

Best Hits

Swiss-Prot: 80% identical to ACND_SHEON: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (acnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 85% identity to abo:ABO_1431)

MetaCyc: 80% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• glyoxylate cycle (5/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle II (plants and fungi) (6/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle I (7/11 steps found)
• TCA cycle III (animals) (6/10 steps found)
• reductive TCA cycle II (5/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.79

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Sequence Analysis Tools

See E4PQ14 at UniProt or InterPro

Protein Sequence (865 amino acids)

>HP15_1930 aconitate hydratase 1 (Marinobacter adhaerens HP15)
MNTEYRKSLPGTDLDYFDTRQAVEDIQAGAYDKLPYTSKILAEQLVRRCDPEVLTDSLKQ
LIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEKGGDPAKVNPVVPTQLIVDH
SLAVEHAGFEKDAFEKNREIEDRRNDDRFHFINWTKTAFKNVDVIPPGNGIMHQINLEKM
SPVVQNRDGVAFPDTCVGTDSHTPMVDALGVISVGVGGLEAESVMLGRASMMRLPDIVGV
ELTGKLQPGITSTDMVLAITEFLRKEKVVGAYLEFYGEGADSLSVGDRATISNMTPEYGA
TAAMFYIDGQTIDYLKLTGREDDQVALVEKFAKETGLWADSMKNAEYERVLKFDLSKVTR
TLAGPSNPHAHLPTSELAARGIAGEWEQEEGKMPDGACIIAAITSCTNTSNPRNMVAAGL
IARNANKLGLTRKPWVKTSLAPGSKTVKMYLEEAKLLPELEQLGFGVVAFACTTCNGMSG
ALDPKIAQEIMDRDLFSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE
KDALGYDQDGNPVTLKDIWPDDAEIDAIVKSSVKPEQFRSTYIPMFDITRDAQANTNPLY
KWRPQSTYIRRPPYWEGGMVGEKTLKGMRPLAVLPDNITTDHLSPSNAIMMNSAAGEYLH
KMGVPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVRDENGNVKQGSLARIEPEGKVVR
MWEAIETYMERKQPLIIIAGADYGQGSSRDWAAKGVALAGVEAIVAEGFERIHRTNLIGM
GVMPLQFEEGTTRKTLGIDGTETYDVEGTPAPRAELTLVIHRKNGSTERIPVVCRLDTAE
EVSIYSAGGVLQRFAEDFLQSEGAA