Protein Info for Psest_1982 in Pseudomonas stutzeri RCH2

Annotation: DNA gyrase, A subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 639 (632 residues), 978.8 bits, see alignment E=9.5e-299 PF00521: DNA_topoisoIV" amino acids 32 to 508 (477 residues), 535.5 bits, see alignment E=1e-164 PF03989: DNA_gyraseA_C" amino acids 540 to 586 (47 residues), 32.4 bits, see alignment 5.2e-12 amino acids 591 to 638 (48 residues), 42.3 bits, see alignment 4.2e-15 amino acids 687 to 730 (44 residues), 38.5 bits, see alignment 6e-14 amino acids 734 to 779 (46 residues), 58.1 bits, see alignment 4.5e-20 amino acids 785 to 829 (45 residues), 35.1 bits, see alignment 7e-13 amino acids 835 to 881 (47 residues), 49.9 bits, see alignment 1.8e-17

Best Hits

Swiss-Prot: 86% identical to GYRA_PSEAE: DNA gyrase subunit A (gyrA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 85% identity to avn:Avin_15810)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKM4 at UniProt or InterPro

Protein Sequence (936 amino acids)

>Psest_1982 DNA gyrase, A subunit (Pseudomonas stutzeri RCH2)
MGELAKEILPVNIEDELKQSYLDYAMSVIIGRALPDARDGLKPVHRRVLFAMSELGNDWN
KPYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSVDGDSAAAM
RYTEVRMAKLAHELLADLEKETVDWVPNYDGTEQIPAVMPTKVPNLLVNGSSGIAVGMAT
NIPPHNLGEVIDGCLALIDNADITIDELMQFIPGPDFPTAGIINGRAGIIEAYRTGRGRI
YIRARADIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKIEGITELRDESDKD
GMRVVIELRRGEVPEVILNNLYAQTQLQSVFGINVVALIDGQPRTLNLKELLEAFVRHRR
EVVTRRTVYELRKARERGHILEGQAVALSNIDPVIAVIKASPTPAEAKDALISTAWESSA
VEAMVERAGADACRPEGLDPQFGLRDGKYFLSPEQAQAILDLRLHRLTGLEHEKLLSEYQ
EILTQIGELIRILTNPVRLMEVIREELEGVKRDFGDARRTEILESRLDLTLADLITEEER
VVTISHGGYAKSQPLAAYQAQRRGGRGKAATGVKEEDYVEHLLVANSHTTLLLFSSKGKV
YWLKTYEIPEASRTSRGRPLVNLLPLDEGERITAMLQVDLEALRKQAAEGEDDLDDAEGL
VIEHDDADEVESDDADSDEKDEPTGSYIFMATANGTVKKTPLVQFSRPRTSGLIALKLEE
GDTLIAAAVTDGAQEVMMFSDGGKVIRFKESKVRIMGRNARGVRGMRLPEGQRIISMLIP
ETGAQILTASVRGYGKRTAMEEFPRRGRGGQGVIAMVTNERNGPLVGAVQVLEGEEIMLI
SDQGTLVRTRVDEVSSLGRNTQGVTLIKLSKGEHLVGLERVQEPSEEDVLEDIEALLDDD
ALEKEMPVLSTEPSEDELLGGAGDVSPDVVPTDDED