Protein Info for Psest_1973 in Pseudomonas stutzeri RCH2

Annotation: phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 791 (791 residues), 860.3 bits, see alignment E=7.6e-263 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 73.3 bits, see alignment E=3.5e-24 PF03483: B3_4" amino acids 211 to 383 (173 residues), 242.3 bits, see alignment E=5.8e-76 PF03484: B5" amino acids 405 to 471 (67 residues), 72.6 bits, see alignment E=6e-24 PF17759: tRNA_synthFbeta" amino acids 474 to 683 (210 residues), 219.6 bits, see alignment E=8.8e-69 PF03147: FDX-ACB" amino acids 698 to 791 (94 residues), 104.6 bits, see alignment E=7.6e-34

Best Hits

Swiss-Prot: 78% identical to SYFB_PSEF5: Phenylalanine--tRNA ligase beta subunit (pheT) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 89% identity to psa:PST_2364)

MetaCyc: 54% identical to phenylalanine--tRNA ligase subunit beta (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMH0 at UniProt or InterPro

Protein Sequence (792 amino acids)

>Psest_1973 phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial (Pseudomonas stutzeri RCH2)
MKFSEQWLRSWVNPQVSREELVARLSMVGLEVDAVTPVAGSFSGVVVGEVLETAQHPDAD
KLRVCQVSNGSETFQVVCGAPNVRPGLKIPFAMIGAQLPGDFKIKKAKLRGIESNGMLCS
ETELQVGSDDSGLMELACDAPVGTDFREFLGLDDVSIEIGLTPNRGDCLSIAGLAREVGA
IYGVAVSPVDVAAVPVGHDDSRPVEVFAPAACPRYLGRIIRDVDLSRPTPMWMVERLRRS
DIRSIDAVVDVTNYVMLELGQPLHAFDLAEINGGIRVRMAEEGEKLVLLDGQEITLRADT
LVIADQQRALAIAGVMGGEHSGVTASTQSIFLESAFFDTIALAGKARSYGLHTDASHRYE
RGVDSQLARQAMERATGLLLAIVGGEAGPIVEVVSESNLPKVTPVTLRTERITQMLGLEM
DGTEVVRLLTSLGLAVTEEAEGRWQVSVPSHRFDIGLEVDLIEELGRLYGYDRLPVRYPQ
ARLAPEAKPEARAELPLLRRLLVARGYQEAITYSFIDPKLFELFSPGVKPLELANPISAD
MAAMRASLWPGLVKALQYNLNRQQSRVRLFEAGLRFVGQLPELEQEAMLAGVVTGSREPE
GWTNVREAVDFFDVKADVEALLANAGNAGAYRFVAGEHPALHPGQTARVEREGTLVGFVG
SLHPELAGKLGIDQPVFMFELKLSEIAEGRMPSFAELSRFPEVRRDLAVLVAREVPAEEI
LTCIRHAAGDYLTDLKLFDVYQGKGIDPLSKSVAVGLTWQHPSRTLNDDEVNGSMQKILT
SLEERFNATLRK