Protein Info for Psest_1961 in Pseudomonas stutzeri RCH2

Annotation: molybdopterin-guanine dinucleotide biosynthesis protein A, proteobacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR02665: molybdenum cofactor guanylyltransferase" amino acids 9 to 191 (183 residues), 190 bits, see alignment E=2e-60 PF12804: NTP_transf_3" amino acids 12 to 167 (156 residues), 124 bits, see alignment E=3.4e-40

Best Hits

Swiss-Prot: 67% identical to MOBA_PSEAB: Molybdenum cofactor guanylyltransferase (mobA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K03752, molybdopterin-guanine dinucleotide biosynthesis protein A (inferred from 73% identity to psa:PST_2376)

Predicted SEED Role

"Molybdopterin-guanine dinucleotide biosynthesis protein MobA" in subsystem Molybdenum cofactor biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMG0 at UniProt or InterPro

Protein Sequence (202 amino acids)

>Psest_1961 molybdopterin-guanine dinucleotide biosynthesis protein A, proteobacterial (Pseudomonas stutzeri RCH2)
MIAPAFSSCSILLLAGGRGQRMGGQDKGLINWHGRPLIEWMHDVVRPLTDELLISCNRNQ
SRYAMYADRLVADEAEGFPGPLAGIRAGLAAARNRWVLLLPCDTPLIDRPLLQSLYSTAI
ETPDRPLMLRSGDQWEPLFSIIPISLRGQIEACWHRGERSPQRVLLQLGAAELLIQPGDP
RLANLNTPELLEMARASTGTSS