Protein Info for Psest_1950 in Pseudomonas stutzeri RCH2

Annotation: D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02113: Peptidase_S13" amino acids 33 to 456 (424 residues), 272 bits, see alignment E=9.2e-85 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 65 to 456 (392 residues), 195.6 bits, see alignment E=5.7e-62

Best Hits

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 94% identity to psa:PST_2387)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMF2 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Psest_1950 D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family (Pseudomonas stutzeri RCH2)
MNRILRGITFAILALPVTLPAVAAGVNQTLPTRVEQALKANKINNDALSVVMLPLTGKAA
PTFINADVSVNPASTMKLVTTYAALELLGPNHQWKTEFYADGPLVDGTLNGNLYLKGGGD
PKLNMEKLWLLLRDLRANGVNKVTGDLVLDRSHFVQPELPVFDDDGGDQNKPFLVGPDSL
LVNLKALRFIVRNDGGKVNIAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV
TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWAELGGSILGRDRVAQVPSSARLLAR
AYSPDLTEIIRDINKYSNNTMAKQLFLSLGATFRNEADADDAKAAQRVIRQWLAKKGLIS
PHLVIENGSGLSRAERVSAREFGNLLQAAWRSPYAAEFISSMPLAAVDGTMRKRLRNTPV
AGKAHVKTGTLNNVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRSE
STAQR