Protein Info for Psest_1949 in Pseudomonas stutzeri RCH2

Annotation: Predicted N6-adenine-specific DNA methylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 PF22020: RlmL_1st" amino acids 6 to 61 (56 residues), 56.7 bits, see alignment 5.7e-19 PF02926: THUMP" amino acids 71 to 156 (86 residues), 58.8 bits, see alignment E=1.7e-19 PF01170: UPF0020" amino acids 165 to 380 (216 residues), 144.3 bits, see alignment E=1.2e-45 PF10672: Methyltrans_SAM" amino acids 487 to 680 (194 residues), 69.6 bits, see alignment E=8.5e-23 PF03602: Cons_hypoth95" amino acids 560 to 652 (93 residues), 29.2 bits, see alignment E=2.1e-10

Best Hits

Swiss-Prot: 95% identical to RLMKL_PSEU5: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 95% identity to psa:PST_2388)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIE3 at UniProt or InterPro

Protein Sequence (726 amino acids)

>Psest_1949 Predicted N6-adenine-specific DNA methylase (Pseudomonas stutzeri RCH2)
MTDRHELILTCPKGLEGLLLEEATALGLEEAREQTSAIRGFGALEIAYRLCLWSRLANRV
LLVISRFPTVDAETLYQGVHAVDWADHLQPAGSLAVEFSGRGSGIDNTHFGALKVKDAIV
DRLRTASGERPSIDKLDPDLRVHLRLDKGQAVLSLDLSGHSLHQRGYRLQQGAAPLKENL
AAAILIRAGWPRIAAAGGALTDPMCGVGTFLVEGAMMAADIAPNLKRERWGFSAWLGHVP
AIWTRLHAEAVARAETGLAKPPLWIRGYEADPRLIQPGRNNIERAGLASWIKVYQGDVGD
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLGWEAAVFTAAPDLGK
RMGIRSHKQYAFWNGALPCKLLLIKVELDQFVTGQRGAASEESTQQQAPVEQARLSEGGQ
MFANRLQKNLRQLGKWARREGIECYRLYDADMPEYALAIDLYRDWVHVQEYAPPRSIDPD
KAQARLLDALAAIPQALGIAREKVVVKRRERQSGTRQYERQATQGEFLEVAEGGVKLLVN
LTDYLDTGLFLDHRPLRLRIQREAAGKRFLNLFCYTATATVHAAKGGARSTTSVDLSKTY
LDWARRNLALNGLSDRQRLEQGDVMAWLEEDRGEYDLIFIDPPTFSNSKRMEGVFDVQRD
HLALLDLAMARLAAGGTLYFSNNFRKFVLDAGLSDRYAVEEITASTLDEDFRRNPRIHRA
WKLQAR