Protein Info for Psest_1947 in Pseudomonas stutzeri RCH2

Annotation: dihydroorotate dehydrogenase, subfamily 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 2 to 338 (337 residues), 429.5 bits, E=3.9e-133
• PF01180: DHO_dh
  • amino acids 44 to 335 (292 residues), 268.3 bits, E=3.8e-84

Best Hits

Swiss-Prot: 98% identical to PYRD_PSEU5: Dihydroorotate dehydrogenase (quinone) (pyrD) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 98% identity to psa:PST_2390)

MetaCyc: 57% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

See L0GKJ7 at UniProt or InterPro

Protein Sequence (343 amino acids)

>Psest_1947 dihydroorotate dehydrogenase, subfamily 2 (Pseudomonas stutzeri RCH2)
MYNLARQLLFKLSPETSHELSLELIGAGGRLGLNAMLSKSPASLPVRVMGLDFPNPVGLA
AGLDKNGEAIRGMSQLGFGFIEVGTVTPRPQPGNPKPRIFRLPEAEAIINRMGFNNHGVD
ALLARVEAARFKGVLGINIGKNFDTPVERADEDYLLCLDKVYHHASYVTVNVSSPNTPGL
RSLQFGDSLKKLLDALRLRREDLEIMHGKRVPLAIKIAPDMTDEETALVGEAVFQAGMDA
IIATNTTLGREGVAGLAYADEAGGLSGAPVREKSTHTVRVLAQTLGGRLPIIAVGGITEG
RHAAEKIEAGASLVQLYSGFIYKGPALIREAVDAIAALRSQAR