Protein Info for Psest_0019 in Pseudomonas stutzeri RCH2

Annotation: ectoine/hydroxyectoine ABC transporter solute-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR02995: ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB" amino acids 19 to 283 (265 residues), 383.5 bits, see alignment E=2.6e-119 PF00497: SBP_bac_3" amino acids 44 to 257 (214 residues), 85.8 bits, see alignment E=1.2e-28

Best Hits

KEGG orthology group: K02030, polar amino acid transport system substrate-binding protein (inferred from 92% identity to psa:PST_0017)

Predicted SEED Role

"amino acid ABC transporter, periplasmic amino acid-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFU2 at UniProt or InterPro

Protein Sequence (285 amino acids)

>Psest_0019 ectoine/hydroxyectoine ABC transporter solute-binding protein (Pseudomonas stutzeri RCH2)
MRNSTIPTRSRRLLIGCAALASLGFASITQADTLEKIKNSSSVRIGYANETPFAYTALDG
SVTGESPEIVRKVFERMEIDTIKPVLTEWGSLIPGLRAGRFDLIAAGMYITPERCKQVLF
TDPHYRLLDTLLVAEGNPKNLRSYEDIAGNPEVKIAIMSGTVNLGYARKAGIKDSQILQV
PDTTSQLQAVRSGRADAAIGTQLTMKGLADKAGDDVEAIADFKDDPSRAGYGALAFRPQD
KALRDAINAELKQWLGSEEHLKTIEPFGFDRSNVTDKTAAELCGA