Protein Info for Psest_1926 in Pseudomonas stutzeri RCH2

Annotation: Uncharacterized anaerobic dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 901 PF04879: Molybdop_Fe4S4" amino acids 4 to 51 (48 residues), 60.6 bits, see alignment 2.3e-20 PF00384: Molybdopterin" amino acids 62 to 487 (426 residues), 245.2 bits, see alignment E=2.1e-76 PF01568: Molydop_binding" amino acids 586 to 696 (111 residues), 85.9 bits, see alignment E=3.9e-28 PF04324: Fer2_BFD" amino acids 847 to 893 (47 residues), 35.5 bits, see alignment 2e-12

Best Hits

KEGG orthology group: None (inferred from 95% identity to psa:PST_2411)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKI0 at UniProt or InterPro

Protein Sequence (901 amino acids)

>Psest_1926 Uncharacterized anaerobic dehydrogenase (Pseudomonas stutzeri RCH2)
MRMTTASTCCYCGVGCGVLIEHDGERILDVAGDPSHPANFGKLCSKGSTLHLTGDLDARA
LYPELRLGKGLARSRTDWDSALEHAASVFAETIREHGPDSVAFYVSGQLLTEDYYAFNKM
ARALVGTNNLDSNSRLCMSSAVVGYKRSLGADAPPCSYEDIELADCLLIAGSNMAYAHPV
LFRRLEEAKAKRPEMTIIVVDPRRTDTSELADLHLPILPGTDVALFHGLLHILMWEGWID
RRFIDGHTEGFDALKSLVRDYTPAMVAELCGISVDDLQTCARLIGSAPAFLSLWCMGLNQ
STAGSAKNSALINLHLATGQIGKPGAGPFSLTGQPNAMGGRETGSLSNLLPGHRDATNAE
HRAEVAHYWGIEQLPKQPGLSAIELFEAVRAGKIKALWIACTNPAQSLPDQQKVHEALAN
CPFVVVQEAFFTTETCRHADLLLPAASWGEKEGTVTNSERRISHVRRAVPAPGEARADWA
ITCDFARRLERHLRPGAPSLFAFENAEALFEEYKPLTAGRDLDYSGLSYALIDTLGPQQW
PFPPGSEQGTAQLYGDGVFPTANGRARFIAEHYQAPKEKREARYPLTLNTGRLRDQWHGM
SRTGTAARLFGHVEEALLGMNAEDMRRRRLLDGQLVTVKSRRGSLILPVQKDDSLRAGQA
FLPMHWGDRFLKGLGSNVLTLASVDPLSKQPELKHAGIEVEQVELPWQFFALVEGDVQRR
FEALRPLFETFDYASFSLGGRERSALLIRAARDAAPTTAELDAIDSLLGLDEGPVLAYDD
PRRAVGKRVRIEEGRIVALRLAGETAARQWLRGLWESGSADAELRRWLLAPLSAPPGGAT
ISTNRTLCNCMNVSQSKVRAGIERGLDLAGLKQELGCGTSCGSCVPEIKRLLASQPERAI
S