Protein Info for Psest_1922 in Pseudomonas stutzeri RCH2

Annotation: aminopeptidase N, Escherichia coli type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 886 TIGR02414: aminopeptidase N" amino acids 13 to 885 (873 residues), 1356 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 110 to 193 (84 residues), 56.7 bits, see alignment E=7.1e-19 PF01433: Peptidase_M1" amino acids 233 to 446 (214 residues), 191.9 bits, see alignment E=2.3e-60 PF11940: DUF3458" amino acids 451 to 555 (105 residues), 109.7 bits, see alignment E=1.6e-35 PF17432: DUF3458_C" amino acids 560 to 886 (327 residues), 418.2 bits, see alignment E=5.1e-129

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 95% identity to psa:PST_2415)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL18 at UniProt or InterPro

Protein Sequence (886 amino acids)

>Psest_1922 aminopeptidase N, Escherichia coli type (Pseudomonas stutzeri RCH2)
MRTEQPKVIHLKDYQAPEYLIDETHLTFELYEDRTLVHAQLVMRRNPGRPAGKLPPLDLH
GQELELLSIALNDRELAAGDYQLSEDCLTLQPDSDSFVIDSSVVIHPESNTALEGLYKSG
SMFCTQCEAEGFRKITYYLDRPDVMSKFTTTVSAEQKAYPVLLSNGNPIASGSEDNGRHW
ATWEDPFKKPAYLFALVAGDLWAIEDSFTTMSGRDVALRIYVEPENVDKCQHAMDSLKKS
MKWDEEAYGREYDLDIFMIVAVNDFNMGAMENKGLNIFNSSAVLARAETATDAAHQRVEA
IVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDSQFSADMNSATVKRVEDVAYLRTHQF
AEDAGPMAHSVRPESFIEISNFYTLTVYEKGAEVVRMIQTLLGEEGFRKGSDLYFERHDG
QAVTVDDFVKAMEDANGADLTQFKRWYSQSGTPRLAVSEQYDEAAKTYSLTFRQSCPPTP
GQPTKEPFVIPVKLGLLAADGSDMPLRLDGEATAVGTSRVLAVTEAEQTFTFVDVAERPQ
PSLLRGFSAPVKLDYPYSRDQLMFLMQHDSDGFNRWEAGQQLSVQVLQELIVQHQRGEAL
AMDERLVEALRSLLQNETLDAAMVAEMLSLPGEAYLTEISEVADVDAIHAAREFARKRIA
DALFEPLWQRYQANRETSRNTAYFASAEHFARRALQNIALSYLMLSEKAEVVAACLEQFE
QADNMTERLTALAVLVNSPFEAERDKALQTFAEHFKDNPLVMDQWFSVQAGSPLPGGLER
VQTLMQHSAFTLKNPNKVRALIGAFANQNLVNFHRADGAGYRFLADQVITLNALNPQIAS
RLLAPLTRWRKYDSARQALMKGELERILASGELSSDVYEVVSKSLA