Protein Info for Psest_0189 in Pseudomonas stutzeri RCH2

Annotation: Negative regulator of beta-lactamase expression

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01510: Amidase_2" amino acids 34 to 165 (132 residues), 110.1 bits, see alignment E=1e-35 PF01471: PG_binding_1" amino acids 200 to 253 (54 residues), 33.4 bits, see alignment E=4.2e-12

Best Hits

KEGG orthology group: K11066, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 87% identity to psa:PST_4056)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHA3 at UniProt or InterPro

Protein Sequence (262 amino acids)

>Psest_0189 Negative regulator of beta-lactamase expression (Pseudomonas stutzeri RCH2)
MKAITAILMLLLLAGCASGPRIDTSHSSIGHDSRSQFIVLHYTSADLPRSLELLSGRDVS
SHYLIGESPATIYRLVDENRRAWHAGESEWNDRTWLNATSIGIELVNRGYVESADGRRLW
HPYSDAQIDALVVLLKDIMARHELKPGAIIGHSDIAPQRKVDPGPLFPWKRLADEGLVPW
PEAGAVAVEQMRYASEGLPPVVWYQDALVAQGYRVPRHGHLDAETRNVIAAFQMKYRPAR
FDGEPDAETAAMLQVLANQAKR