Protein Info for Psest_1917 in Pseudomonas stutzeri RCH2
Annotation: Predicted sugar kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to NADK_PSEE4: NAD kinase (nadK) from Pseudomonas entomophila (strain L48)
KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 99% identity to psa:PST_2420)Predicted SEED Role
"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)
MetaCyc Pathways
- NADP biosynthesis (1/1 steps found)
- NAD phosphorylation and transhydrogenation (1/2 steps found)
- cytosolic NADPH production (yeast) (3/5 steps found)
- mitochondrial NADPH production (yeast) (3/5 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (5/8 steps found)
- NAD phosphorylation and dephosphorylation (1/3 steps found)
- NAD(P)/NADPH interconversion (3/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.23
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GL08 at UniProt or InterPro
Protein Sequence (295 amino acids)
>Psest_1917 Predicted sugar kinase (Pseudomonas stutzeri RCH2) MEQFRNIGIIGRLGSSQVLDTIRRLKRFLVERHLHVILEENIAEVLPGHGMQTSSRQRLG ESCDLVIVVGGDGSLLGAARAMAKHRVPVLGINRGSLGFLTDIRPDELEEKVAEVLNGQY TLENRFLLEAQARRFDEPIGEGDALNDVVLHPGKSTRMIEFELYIDGQFVCSQKADGLIV ATPTGSTAYSLSAGGPIMHPRLDAIVVVPMYPHTLSSRPIVVDGNSELKIVVSPNMQIYP QVSCDGQNHFTCAPGDTVTVRKKPQKLHLIHPLDHNYYEVCRTKLGWGSRLGGGD