Protein Info for Psest_1911 in Pseudomonas stutzeri RCH2

Annotation: spermidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00417: spermidine synthase
  • amino acids 4 to 275 (272 residues), 343.4 bits, E=4.3e-107
• PF17284: Spermine_synt_N
  • amino acids 10 to 54 (45 residues), 57.1 bits, 2e-19
• PF01564: Spermine_synth
  • amino acids 57 to 235 (179 residues), 220.5 bits, E=2e-69
• PF08241: Methyltransf_11
  • amino acids 81 to 186 (106 residues), 21.5 bits, E=4.5e-08

Best Hits

Swiss-Prot: 82% identical to SPEE_PSESM: Polyamine aminopropyltransferase (speE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00797, spermidine synthase [EC: 2.5.1.16] (inferred from 89% identity to psa:PST_2426)

MetaCyc: 60% identical to spermidine synthase (Escherichia coli K-12 substr. MG1655)
Spermidine synthase. [EC: 2.5.1.16]; 2.5.1.- [EC: 2.5.1.16]

Predicted SEED Role

"Spermidine synthase (EC 2.5.1.16)" in subsystem Polyamine Metabolism (EC 2.5.1.16)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• aminopropylcadaverine biosynthesis (2/3 steps found)
• spermidine biosynthesis I (1/2 steps found)
• superpathway of polyamine biosynthesis I (5/8 steps found)
• superpathway of polyamine biosynthesis II (5/8 steps found)
• L-methionine salvage cycle III (6/11 steps found)
• L-methionine salvage cycle I (bacteria and plants) (5/12 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutathione metabolism
• Methionine metabolism
• Urea cycle and metabolism of amino groups
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.16

Use Curated BLAST to search for 2.5.1.16

Sequence Analysis Tools

See L0GKE6 at UniProt or InterPro

Protein Sequence (286 amino acids)

>Psest_1911 spermidine synthase (Pseudomonas stutzeri RCH2)
MSDFQETLFEGYGQRFQIDKLLHEVRTEHQHLVIFENARMGRVMALDGVIQTTEADEFIY
HEMLAHVPILAHGLARRVLIIGGGDGGVLREVARHRDIESITMVEIDGAVVETCREFLPN
HSAGTFDDPRLKLVIADGMHFVTNTEEKFDVIISDSTDPIGPAEVLFSEHFYQACRRCLN
DGGILVTQNGTPFMQLGEVQTTAKRLHGLFADWHFYQAAVPTYLGGAMAFAWGACNAESR
KLPLETLLQRFVGSGIVTRYYNPHVHIAAFALPQYVLHAIAKPSND