Protein Info for Psest_0183 in Pseudomonas stutzeri RCH2

Annotation: glycine dehydrogenase (decarboxylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 PF02347: GDC-P" amino acids 18 to 441 (424 residues), 601.5 bits, see alignment E=9.1e-185 amino acids 462 to 729 (268 residues), 29.5 bits, see alignment E=3.8e-11 TIGR00461: glycine dehydrogenase" amino acids 19 to 950 (932 residues), 1535.5 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 779 to 900 (122 residues), 196.6 bits, see alignment E=1.1e-62

Best Hits

Swiss-Prot: 85% identical to GCSP2_PSEPF: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 69% identity to aha:AHA_1720)

MetaCyc: 65% identical to glycine decarboxylase (Escherichia coli K-12 substr. MG1655)
Glycine dehydrogenase (decarboxylating). [EC: 1.4.1.27, 1.4.4.2]

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHK2 at UniProt or InterPro

Protein Sequence (958 amino acids)

>Psest_0183 glycine dehydrogenase (decarboxylating) (Pseudomonas stutzeri RCH2)
MPYMPSLSQLQQPDAFLRRHLGPDQAEQQAMLDALGLTSREQLIEQTVPPAIRLQDELDL
PAALDEQAALAKLRGYAEQNQLWTSLIGMGYHGTITPPVILRNVLENPGWYTAYTPYQPE
IAQGRLEALLNYQQMIIDLTGLDLANASLLDEATAAAEAMTLARRMAKSKSNRFFVDENC
HPQTLSVVQTRAEAFGFELVVGTLDELAGQEVFGALLQYPDTHGEIRDLRPAIEQLHAQQ
ALACVAADLLSLLLLTPPGELGADVVLGSTQRFGVPMGYGGPHAAYFASRDEFKRGMPGR
IIGVSKDARGNTALRMALQTREQHIRREKANSNICTAQVLLANIAGFYAVYHGPQGLKRI
AQRVHRLTAILAAGLEQKGIVRLNQHFFDTLTLEVGGAQTAIIESAEAAQINLRILGRGR
LGVSLDETCDERTVEQLLAIFLGADHGLDVAALDAGELAAGIPAGLQRESGYLEHPVFNS
HHSETEMLRYLKQLENKDLALNQAMIPLGSCTMKLNATSEMIPITWAEFANLHPFVPRVQ
AQGYKLMIEELEAWLCAITGFDAISMQPNSGAQGEYAGLVAIRKYHESRGEGQRDICLIP
SSAHGTNPASAQMVSMRVVIVECDKGGNVDLEDLKRKAAEAGDRLSCLMITYPSTHGVYE
ENVREICAAIHAHGGQVYMDGANLNAQVGLARPADIGADVSHMNLHKTFCIPHGGGGPGM
GPIGVKAHLAPFVANHPVVELEGPQPGNGAVSAAPWGSASILPISWMYIAMMGPQLRDAT
EVAILGANYLANRLGDAFPVLYAGRNGRVAHECILDLRPLKAASGISEEDVAKRLMDYGF
HAPTMSFPVPGTLMIEPTESESKAELDRFVEAMLSIRAEIAKVQDGEWPADNNPLVRAPH
TLADVIGEWDRPYSIAEAVTPSAHARAHKYWPAVNRVDNVYGDRNLFCACVPVDAYRD