Protein Info for Psest_1828 in Pseudomonas stutzeri RCH2

Annotation: polyphosphate:AMP phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR03708: polyphosphate:AMP phosphotransferase" amino acids 2 to 494 (493 residues), 726.8 bits, see alignment E=1.8e-222 PF03976: PPK2" amino acids 11 to 234 (224 residues), 166.8 bits, see alignment E=3e-53 amino acids 271 to 495 (225 residues), 299.4 bits, see alignment E=8.8e-94

Best Hits

Swiss-Prot: 72% identical to PK22_PSEAE: Polyphosphate:AMP phosphotransferase (PA3455) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 94% identity to psa:PST_2503)

Predicted SEED Role

"UDP-galactose-lipid carrier transferase (EC 2.-.-.-)" (EC 2.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM16 at UniProt or InterPro

Protein Sequence (499 amino acids)

>Psest_1828 polyphosphate:AMP phosphotransferase (Pseudomonas stutzeri RCH2)
MFESAEIGHSIDKATYDAAVPELREALLQAQYRLKAQAGFPVLILINGIEGAGKGETIKL
LNEWMDPRLIRVDSFDVPSDEELAHPPAWRFWRRLPPKGHTGIFFGNWYSKMLEDRVHGR
INGAELKLATARAQRLERMLCDEGALIFKFWFHLSKERMNARLDSLKNDPLHSWRISPLD
WQQSKTYDKFVHHGEKILRLSSRDFAPWYVIEGADERYRSLTVGRILLEGLQAALAAAEQ
PERASSQPHSAPLQIDLDHLSLLDSLDLSQKLSKDDYQQQLAEEQARLSQLLRDRRLRKR
GVLALFEGHDAAGKGSAIRRVTGALDPRMYRTIQIAAPSEEERAQPWLWRFWRNVPERGK
FTIFDRSWYGRVLVERVEGFCTPDEWLRAYSEINDFEEQLVDADIVLVKFWLTIDKNTQL
KRFKEREETVFKRFKITDEDWRNRERWDDYGHAVADMVDRTSTEIAPWTLVEANDKRFAR
VKVLRTLSEALEAAIEKAR