Protein Info for Psest_1804 in Pseudomonas stutzeri RCH2

Annotation: Superfamily II DNA and RNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00270: DEAD" amino acids 30 to 195 (166 residues), 172.2 bits, see alignment E=1.6e-54 PF04851: ResIII" amino acids 42 to 189 (148 residues), 26.1 bits, see alignment E=1.5e-09 PF00271: Helicase_C" amino acids 231 to 340 (110 residues), 99.6 bits, see alignment E=2.5e-32 PF03880: DbpA" amino acids 486 to 551 (66 residues), 66 bits, see alignment E=5e-22

Best Hits

KEGG orthology group: K05592, ATP-dependent RNA helicase DeaD [EC: 3.6.4.13] (inferred from 97% identity to psa:PST_2507)

Predicted SEED Role

"DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLU0 at UniProt or InterPro

Protein Sequence (556 amino acids)

>Psest_1804 Superfamily II DNA and RNA helicases (Pseudomonas stutzeri RCH2)
MTQEIGGFAALGIHSAVLAAISAVGYEEPSPIQSQAIPVILGGHDMIGQAQTGTGKTAAF
ALPILSKIDPARKEVQALILAPTRELALQVATAFETYSKQMPGLTVVAVYGGAPMGPQLK
AIRQGAQVIVATPGRLVDHLSRNSGLLSTIRFLVLDEADEMLKLGFMDDLEVIFEAMPES
RQTVLFSATLPHSIRAIAEKHLREPQHIKIAAKTQTVARIEQAHLMVHADQKIQAVLRLL
EVEDFDALIAFVRTKQATLDLAAALEAKGYKAAALNGDIAQNQRERVIESLKDGRLDIVV
ATDVAARGLDVARITHVFNVDMPYDPESYVHRIGRTGRAGREGRALLLVTPRERRMLQVI
ERVTNQKVAEARLPNAQQVLDARIKKLTNSLAPLVADAEATHGELLDKLVADIGCSPRAL
AAALLRKATNGQALTLAEVEREQPLVPTSSPRERTERSDRPERSGDRERRAPVPLAEGRA
RCRTPLGARDGIAARNLLGAILNEGGLAREAIGRIQVRDSFSLVELPEDGLERLLGKLKD
TRVGGKQLKLRRYRED