Protein Info for HP15_1712 in Marinobacter adhaerens HP15

Annotation: glycogen branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 26 to 641 (616 residues), 940.7 bits, see alignment E=1.9e-287 PF02922: CBM_48" amino acids 42 to 124 (83 residues), 85.7 bits, see alignment E=3.4e-28 PF00128: Alpha-amylase" amino acids 193 to 266 (74 residues), 48.6 bits, see alignment E=1.4e-16 PF02806: Alpha-amylase_C" amino acids 546 to 642 (97 residues), 84.9 bits, see alignment E=6.1e-28

Best Hits

Swiss-Prot: 80% identical to GLGB_MARHV: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 80% identity to maq:Maqu_1434)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PN27 at UniProt or InterPro

Protein Sequence (644 amino acids)

>HP15_1712 glycogen branching enzyme (Marinobacter adhaerens HP15)
MKGRPKRLIWSGTARSMDSPTLSEFDLHLFAEGRHWHIYNVLGAHVCRNRGVEGVRFAVW
APHASAVSVAGDFNEWNAGIHPMSLHEGTGVWSCFIPGIGNGALYKFAITTDKGRQILKT
DPYGQAFELRPSTAAVVTERGHFGWGDGDWLARRGRWNWQDSPISIYEVHAASWRHHGSG
RWLTYRELADQLIPYVLELGFTHIELLPVTEHPLDESWGYQTTGYYAPTSRFGTPDDLRY
LVDQCHRHNIGVILDWVPGHFPTDEHALARFDGSALYEHEDPRKGRHQDWGTLIYNYGRH
EVRNFLIGSALFWLDAFHIDGLRVDAVASMLYLNYSRKEGEWEPNVHGGHENLEAIEFLR
ELNQVCQSRFPGTLVCAEESTSWPRVTRPPEIGGLGFNLKWNMGWMHDTLDYLAQDPVYR
QYHHDKLTFGLLYAFSENFLLPLSHDEVVHGKGSLINKMTGDEWQQRATLRVLFTYMFSY
PGAKLLFMGGEFGQRREWSESRELDWSLLAYPEHQGIKKLVQDLNGIYRREVSLHGACFK
PDGFEWIDCHDSPQSVISFLRTAKGETSVIVVNFTPMPRHHYRIGLPEGGNWREIFNSDS
EYYGGSNLGNPYPMPADAQPWMNREWSVELTLPPLGAIILRPAL