Protein Info for Psest_0017 in Pseudomonas stutzeri RCH2

Annotation: ectoine/hydroxyectoine ABC transporter, permease protein EhuD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 55 to 78 (24 residues), see Phobius details amino acids 88 to 102 (15 residues), see Phobius details amino acids 153 to 155 (3 residues), see Phobius details amino acids 189 to 209 (21 residues), see Phobius details TIGR03003: ectoine/hydroxyectoine ABC transporter, permease protein EhuD" amino acids 4 to 214 (211 residues), 340 bits, see alignment E=6.3e-106 TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 13 to 110 (98 residues), 96.5 bits, see alignment E=1.1e-31 PF00528: BPD_transp_1" amino acids 34 to 216 (183 residues), 101.3 bits, see alignment E=2.8e-33

Best Hits

Swiss-Prot: 48% identical to Y4TG_SINFN: Probable amino-acid ABC transporter permease protein y4tG (NGR_a01520) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02029, polar amino acid transport system permease protein (inferred from 97% identity to psa:PST_0015)

Predicted SEED Role

"amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGT0 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Psest_0017 ectoine/hydroxyectoine ABC transporter, permease protein EhuD (Pseudomonas stutzeri RCH2)
MNFFDWQFAREILPRLLDASLTTIGIALAGFAIAVVLGLFLAIGRRSRLRWLSWPITGLI
EFIRSTPLLIQVYFLFYVFPNYGLNLSAMQAGIIGIALHYACYTAEVYRAGLDAVPRSQW
EAVTALNMKPWTAYRDVILPQALRPVLPALGNYLISILKDTPVLSAITVVEIMQRAKNIG
SESFRYLEPITMVGIFFLILSLGLAWCVRRLENRMELTR