Protein Info for Psest_1696 in Pseudomonas stutzeri RCH2

Annotation: hydrolase, TatD family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 TIGR00010: hydrolase, TatD family" amino acids 2 to 254 (253 residues), 272.1 bits, see alignment E=2.3e-85 PF01026: TatD_DNase" amino acids 3 to 254 (252 residues), 273.9 bits, see alignment E=6.6e-86

Best Hits

Swiss-Prot: 36% identical to YABD_BACSU: Uncharacterized metal-dependent hydrolase YabD (yabD) from Bacillus subtilis (strain 168)

KEGG orthology group: K03424, TatD DNase family protein [EC: 3.1.21.-] (inferred from 91% identity to psa:PST_2614)

Predicted SEED Role

"Putative deoxyribonuclease YcfH" in subsystem YcfH

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.-

Use Curated BLAST to search for 3.1.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJR1 at UniProt or InterPro

Protein Sequence (261 amino acids)

>Psest_1696 hydrolase, TatD family (Pseudomonas stutzeri RCH2)
MLVDSHCHLDRLDLTAHGGSLDAALDAARSRGVGHFLCIGVSADNAAAVKALAERYADVD
CSIGVHPLDLEPTSQPVLDWLLGELQHPHVVAIGETGLDYHYEPEAAQMQQQSFRLHLEA
ARLTGKPVIVHTRAARADTLDLLREAALPQAGVLHCFTEDWDMAKAALDLGFYISLSGIV
TFRNADALRDVARKVPADRLLVETDAPYLAPIPHRGKANLPQYVREVAEFLAQLRGVPFE
VLAEQTTANFKTLFPLARVTG