Protein Info for Psest_0162 in Pseudomonas stutzeri RCH2

Annotation: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01676: Metalloenzyme
  • amino acids 10 to 501 (492 residues), 298.9 bits, E=3.2e-93
• TIGR01307: phosphoglycerate mutase (2,3-diphosphoglycerate-independent)
  • amino acids 10 to 512 (503 residues), 769.9 bits, E=4.9e-236
• PF06415: iPGM_N
  • amino acids 88 to 300 (213 residues), 271.7 bits, E=4.4e-85

Best Hits

Swiss-Prot: 86% identical to GPMI_PSEMY: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 97% identity to psa:PST_4080)

MetaCyc: 63% identical to 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Escherichia coli K-12 substr. MG1655)
3PGAREARR-RXN [EC: 5.4.2.12]

Predicted SEED Role

"2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 5.4.2.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• Bifidobacterium shunt (15/15 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• homolactic fermentation (11/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• Rubisco shunt (8/10 steps found)
• glycolysis IV (8/10 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• photorespiration II (6/10 steps found)
• glycerol degradation to butanol (10/16 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.2.1 or 5.4.2.12

Sequence Analysis Tools

See L0GHI2 at UniProt or InterPro

Protein Sequence (515 amino acids)

>Psest_0162 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Pseudomonas stutzeri RCH2)
MSVAPTAAPKPLVLIILDGFGHSDSPDFNAIHAARKPVYDRLLASQPHGLISGSGMDVGL
PDGQMGNSEVGHMNLGAGRVVYQDFTRVTKAIRDGEFFANPTICGAVDKAVGAGKAVHIL
GLLSDGGVHSHQDHLVAMAELAAQRGAEKIYLHAFLDGRDTPPKSAQHSIELLQATFTRL
GKGRIASLIGRYFAMDRDNRWDRVQQAYELIVNGKAEHRSDYAVDGLIAAYERGESDEFV
KATSIGEPVRVEDGDAVVFMNFRADRARELTRCFVEPGFKEFERARVPQLAEFVMLTQYA
ASIPAPAAFAPEALTNVLGEYLANNGKTQLRIAETEKYAHVTFFFSGGREEPFPGEERIL
IPSPQVATYDLKPEMSAPEVTDRIVDAIENQRYDVIVVNYANGDMVGHTGVFEAAVKAVE
CLDSCVGRIVEALDKVGGEALLTADHGNVEQMEDVMTGQAHTAHTCEPVPFVYIGKRNVT
IRQGGVLADVAPTMLTLLGMPVPPEMTGRSIVELS