Protein Info for Psest_1654 in Pseudomonas stutzeri RCH2

Annotation: glyceraldehyde-3-phosphate dehydrogenase, type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00044: Gp_dh_N
  • amino acids 134 to 244 (111 residues), 65.5 bits, E=4.8e-22
• TIGR01534: glyceraldehyde-3-phosphate dehydrogenase, type I
  • amino acids 135 to 468 (334 residues), 371.9 bits, E=1.4e-115
• PF02800: Gp_dh_C
  • amino acids 299 to 456 (158 residues), 148.7 bits, E=1.1e-47

Best Hits

Swiss-Prot: 91% identical to GAP2_PSEAB: Glyceraldehyde-3-phosphate dehydrogenase-like protein (gap2) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 98% identity to psa:PST_2656)

Predicted SEED Role

"NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.13)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• Bifidobacterium shunt (15/15 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• homolactic fermentation (11/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• heterolactic fermentation (15/18 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis IV (8/10 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• oxygenic photosynthesis (11/17 steps found)
• glycerol degradation to butanol (10/16 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.12

Use Curated BLAST to search for 1.2.1.12 or 1.2.1.13

Sequence Analysis Tools

See L0GLK0 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Psest_1654 glyceraldehyde-3-phosphate dehydrogenase, type I (Pseudomonas stutzeri RCH2)
MTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVTSIYGRGLINRSVIAILKAHRFAR
HRIADEAELSVHDTFQILKTMSEMNLGAASVDLGKLVAKYKEAGNGRNLEQFVREELAEV
ADKRHAASGHKGTDVVLYGFGRIGRLLARILIEKTGGGDGLRLRAIVVRKGADNDLVKRA
SLLRRDSVHGPFDGTITIDEENNTITANGNLIQVIYSNDPSAVDYTQYGIENALLVDNTG
KWRDAEGLGQHLKCPGVARVVLTAPGKGELKNIVHGINHGDITADDKIISAASCTTNAIV
PVLKAVNDQYGIVNGHVETVHSYTNDQNLIDNFHKGSRRGRSAALNMVITETGAATAAAK
ALPVLKGKLTGNAIRVPTPNVSMAILNLNLEKATNREEINEYLRQMAMHSDLQKQIDFVN
SQEVVSSDFVGSRHAGVVDAEATICNDNRVVLYVWYDNEFGYSCQVVRVMEDMAGVNPPA
FPR