Protein Info for Psest_0016 in Pseudomonas stutzeri RCH2

Annotation: ectoine/hydroxyectoine ABC transporter, ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 TIGR03005: ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA" amino acids 25 to 275 (251 residues), 444.1 bits, see alignment E=6e-138 PF00005: ABC_tran" amino acids 40 to 199 (160 residues), 125.6 bits, see alignment E=2.3e-40

Best Hits

Swiss-Prot: 67% identical to Y4TH_SINFN: Probable amino-acid ABC transporter ATP-binding protein y4tH (NGR_a01510) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02028, polar amino acid transport system ATP-binding protein [EC: 3.6.3.21] (inferred from 94% identity to psa:PST_0014)

Predicted SEED Role

"amino acid ABC transporter, ATP-binding protein"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH33 at UniProt or InterPro

Protein Sequence (278 amino acids)

>Psest_0016 ectoine/hydroxyectoine ABC transporter, ATP-binding protein (Pseudomonas stutzeri RCH2)
MTTPMQSTDTLNPTPQQPEQAQPLVRFAGVTKRYGELTVLDGLDLQIEEGEKVAIIGPSG
SGKSTLLRVLMTLEGIDEGVIEVDGEPLTHMPDASGQLVPANARHLRRVRGKVGMVFQSF
NLFPHMNALQNVMEAPVQVLGLSKAEARGRAEELLAMVGLEDKLEHFPAQLSGGQQQRVA
IARALAMRPKVMLFDEVTSALDPELCGEVLNVIRRLGEAHNLTMLMVTHQMGFAREFADR
VCFFHQGRIHEQGSPDELFNNPQEERTREFLSAVNEAH