Protein Info for Psest_1627 in Pseudomonas stutzeri RCH2

Annotation: Superfamily II DNA and RNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 PF00270: DEAD" amino acids 24 to 187 (164 residues), 153.8 bits, see alignment E=5.5e-49 PF04851: ResIII" amino acids 39 to 185 (147 residues), 29.7 bits, see alignment E=8.4e-11 PF00271: Helicase_C" amino acids 232 to 338 (107 residues), 100.3 bits, see alignment E=1.2e-32

Best Hits

KEGG orthology group: None (inferred from 88% identity to psa:PST_2682)

Predicted SEED Role

"ATP-dependent RNA helicase SrmB" in subsystem ATP-dependent RNA helicases, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GK65 at UniProt or InterPro

Protein Sequence (443 amino acids)

>Psest_1627 Superfamily II DNA and RNA helicases (Pseudomonas stutzeri RCH2)
MFDQFALHERLLKAVAELKFVEPTPVQVAAIPPALEGRDLRVTAQTGSGKTAAFVLPMLN
RLIGDAVVRTDVRALILLPTRELAQQTLKEVERFSQFTFIKSAMITGGEDFKVQAAVLRK
VPDILIGTPGRMIEHLNAGTLILKDVEMLILDEADRMLDMGFAEDVQRLVGECAKRQQTL
LFSATSGGSALREMTASVLRDPLHLQLNRISELSEGTRQQIITAEDTAHKEKLVHWLLAN
ETYKKAVIFTNTRVQADRLYGRLVAEGVKAFVLHGDKDQKDRKLAIDRVKEGGVKVLVAT
DVAARGLDIEGLDLVINFDMPRSGDEYVHRIGRTGRAGAEGLAISLICHNDWNLMSSIER
YLKQRFEHRVVKGLKGSYMGPKNLKASGKAAGPKKKKDDKKKDDKKKAKPTAKRPKNAKR
EAPSPVVSQDGMAPLKRKKPTAE