Protein Info for Psest_1608 in Pseudomonas stutzeri RCH2

Annotation: glycerol-3-phosphate O-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF27464: PlsB_N
  • amino acids 11 to 212 (202 residues), 224.3 bits, E=1.7e-70
• TIGR03703: glycerol-3-phosphate O-acyltransferase
  • amino acids 14 to 812 (799 residues), 1086.3 bits, E=0
• PF01553: Acyltransferase
  • amino acids 290 to 428 (139 residues), 82.3 bits, E=4.4e-27
• PF19277: GPAT_C
  • amino acids 439 to 780 (342 residues), 391.6 bits, E=5.2e-121

Best Hits

Swiss-Prot: 81% identical to PLSB_PSEMY: Glycerol-3-phosphate acyltransferase (plsB) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 95% identity to psa:PST_2703)

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (3/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

See L0GHE6 at UniProt or InterPro

Protein Sequence (833 amino acids)

>Psest_1608 glycerol-3-phosphate O-acyltransferase (Pseudomonas stutzeri RCH2)
MTRSPIRRLIFSVLRRVLYFWVRSETINQSAFTLKLDRSKPVFYVLQQSSLSDLAVLDVE
CTKAGLPRPVADVAVGEHVEPAAFFFLNPAASWFGRRTRLVAPPALVRLVGALEHNAVEN
AQIIPVSVFWGQSPNRETSAWKLLFADSWAVTGRLRKLLSILVLGRKTRVQFSAPIQLNE
LIALNKGHERTLRMVHRMLRVHFRNQKTAVIGPDLSHRRNLVKGLVHAPQVRQAIRDEAE
RENISIERAEARALRYGNEIASDYAYTAVRFLEVVLSWFWNKIYDGIRVNHIEPLQEAVR
GYEVIYVPCHRSHIDYLLLSYLLFRNGLTPPHIAAGINLNMPVIGSLLRRGGAFFMRRSF
KGNPLYTSVFNEYLHTLFSRGFPVEYFVEGGRSRTGRMLQPKTGMLALTLRSYLRSSRLP
ILFVPVYIGYERVLEGRTYLGELRGAAKKKESIFDIFKVIGALKQRFGQVWVNFGEPLKL
NEFLEQEQPGWRQQSHTPDFRPEWLNDTTNRLAERIAQRLNEAAAVNPVNLVALAMLSTS
RQALDQQSLARILDLYQTLLRAVPYSPHTTLPEGDGNALIAYVKSLDLLAEQKDALGNIL
YLDEQNAVLMTYYRNNVMHIFALPALLASFFQSSSRISREQIQRFTEALYPYLRAELFIR
WEVEELEAVVDQWLAAFVEQGLLKVDGQHYVRPAPSSRQFVLLTLLARVVMQTLQRFYMA
TSLLLNSGQNSLTAEELETLCTVMAQRLSILHGLNAPEFFDKTLFRHFIQSMQEQGVVSP
DENGRLGYHEKLAELAEGAAKRVLPAEIRLSIRQVALERHEDESEPAPPATGA