Protein Info for HP15_1522 in Marinobacter adhaerens HP15

Annotation: dihydrolipoamide acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 75 (72 residues), 60.1 bits, E=2.3e-20
• TIGR01347: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex
  • amino acids 4 to 409 (406 residues), 618.5 bits, E=3.3e-190
• PF02817: E3_binding
  • amino acids 113 to 147 (35 residues), 60.8 bits, 1.8e-20
• PF00198: 2-oxoacid_dh
  • amino acids 178 to 407 (230 residues), 284.4 bits, E=1e-88

Best Hits

Swiss-Prot: 59% identical to ODO2_ECO57: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (sucB) from Escherichia coli O157:H7

KEGG orthology group: K00658, 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) [EC: 2.3.1.61] (inferred from 93% identity to maq:Maqu_1155)

MetaCyc: 59% identical to 2-oxoglutarate dehydrogenase E2 subunit (Escherichia coli K-12 substr. MG1655)
Dihydrolipoyllysine-residue succinyltransferase. [EC: 1.2.1.105, 2.3.1.61]

Predicted SEED Role

"Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61)" in subsystem TCA Cycle (EC 2.3.1.61)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (4/6 steps found)
• TCA cycle II (plants and fungi) (6/9 steps found)
• TCA cycle III (animals) (6/10 steps found)
• 2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA (2/5 steps found)
• L-tryptophan degradation III (eukaryotic) (5/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (5/23 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.105 or 2.3.1.61

Sequence Analysis Tools

See E4PL78 at UniProt or InterPro

Protein Sequence (409 amino acids)

>HP15_1522 dihydrolipoamide acetyltransferase (Marinobacter adhaerens HP15)
MSTEIKAPVFPESVAEGTVATWHKQPGEACSRDELIVDIETDKVVLEVVAPADGVIEEII
KNEGDTVESGEVVGKFKEGAKGESKPAEGKKEESKEEAPKEEAKSEASSGEAILSPAARK
LAEENNVDPNSIKGTGKDGRVTKEDVQNHVDSAKSSGGAAAPQPAAGMPEVNVSQGERPE
KRVPMTRLRASIAKRLVNAQQSAAMLTTFNEVNMGPIMEMRKQYQDSFVKRHGIKLGFMS
FFTKAATEALKRFPAVNASIDGNDMVYHGYQDIGVAVSTDRGLVVPVLRDSDAMGLADIE
KKIVEYGTKAKEGKLAIEDMTGGTFTITNGGIFGSLISTPILNPPQTAILGMHKIQERPM
AVNGKVEIQPMMYLALSYDHRMIDGKEAVQFLVAIKEMLEDPARILLDV