Protein Info for Psest_1585 in Pseudomonas stutzeri RCH2

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 TIGR01891: amidohydrolase" amino acids 36 to 406 (371 residues), 344.9 bits, see alignment E=2.9e-107 PF01546: Peptidase_M20" amino acids 94 to 415 (322 residues), 150.7 bits, see alignment E=5.4e-48 PF07687: M20_dimer" amino acids 214 to 307 (94 residues), 38.6 bits, see alignment E=9.4e-14

Best Hits

Swiss-Prot: 44% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 67% identity to tmz:Tmz1t_1302)

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL58 at UniProt or InterPro

Protein Sequence (423 amino acids)

>Psest_1585 amidohydrolase (Pseudomonas stutzeri RCH2)
MVVTEAKILPSADHHKSYDRHMNTSDLFQPDEAAMKTWRHAIHQNPELGFAEFATSRLVA
DCLETWGYEVHTGIATTGVVGILRWGDGQPRLGLRADMDALPVQELTGLPWASRTPDQMH
ACGHDGHTSILLGAAQSFALMQREGLLGASGTLTLIFQPAEELGGSGGARRMLDEGLLER
FPCDAVFAMHNYPGIPTGHFRFREGPFMASSDRVVIRFNGKGGHGALPHMAIDPMLPAAA
TVLALQSIVGRNVDPVDAAVISVGRIAAGNTYNVIPETAEMELSVRALRPDVRDLLEMRI
RALVEGQAAAFGVTCEVLYERGYPVLINSARETCLAVEAARALVGDERVEVDGAPISGSE
DFAFILQQVPGCYLLIGNGDNGFGGGEHLGPCSVHNPHYDFNDACLAPGAAFWLTLGSRF
FGC