Protein Info for Psest_1569 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF13188: PAS_8" amino acids 9 to 61 (53 residues), 32.6 bits, see alignment 2.5e-11 TIGR00229: PAS domain S-box protein" amino acids 10 to 122 (113 residues), 77.2 bits, see alignment E=1.2e-25 PF00989: PAS" amino acids 11 to 110 (100 residues), 53 bits, see alignment E=1.5e-17 PF08448: PAS_4" amino acids 17 to 119 (103 residues), 56.7 bits, see alignment E=1.2e-18 PF13426: PAS_9" amino acids 18 to 116 (99 residues), 55 bits, see alignment E=3.8e-18 PF08447: PAS_3" amino acids 29 to 98 (70 residues), 52.3 bits, see alignment E=2.6e-17 PF13185: GAF_2" amino acids 138 to 272 (135 residues), 38.6 bits, see alignment E=5.6e-13 PF01590: GAF" amino acids 139 to 271 (133 residues), 36.6 bits, see alignment E=2.8e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 280 to 441 (162 residues), 146.6 bits, see alignment E=5.6e-47 PF00990: GGDEF" amino acids 284 to 440 (157 residues), 170 bits, see alignment E=1.6e-53

Best Hits

KEGG orthology group: None (inferred from 88% identity to psa:PST_2732)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLB6 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Psest_1569 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MKTDSCAPLASVIDLLLDAICVVDAQGNIVYVNAACERIFGYTPEEMVGRRMLEMVYPED
RARTVAAAHEVMASQPLLHFENRYVRKDGSVAHIMWSACWSTDNKLRIGVARDITQRKQS
EAMQAALYSISEAAHGAKDLAALYQQIHLTIARLLPVDDFIVAIRNAASGELTYPYQALA
LDSTGLPAEPAASLCFSVLHGAQPLRFNADSMRALALPLGDGDIRAWWLGVPLNSSEGVI
GVLLLKSTAVPYTEKDQELLQFVSTQVVTAIERQQLYARLHRMAQYDELTGLPNRACFRD
RLSTALARVRRNGGRIALLYVDLDRFKQINDTYGHGGGDQLLRAVADRLTACVRDTDTVA
RLGGDEFVVLLESIAEPEDAQRVVEKVRSAFEQPLSITGHSLEIGMSIGIALYPEDGETE
TQLLKQADDRMYLMKARGGQQSVDCD