Protein Info for Psest_0153 in Pseudomonas stutzeri RCH2

Annotation: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 PF07685: GATase_3" amino acids 4 to 109 (106 residues), 28.6 bits, see alignment E=1.1e-10 TIGR01855: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit" amino acids 4 to 207 (204 residues), 220.4 bits, see alignment E=9.9e-70 PF00117: GATase" amino acids 6 to 205 (200 residues), 69.2 bits, see alignment E=3.9e-23

Best Hits

Swiss-Prot: 92% identical to HIS51_PSEAE: Imidazole glycerol phosphate synthase subunit HisH 1 (hisH1) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 92% identity to pfl:PFL_0362)

Predicted SEED Role

"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH65 at UniProt or InterPro

Protein Sequence (212 amino acids)

>Psest_0153 imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (Pseudomonas stutzeri RCH2)
MQTVAVIDYGMGNLHSVAKALEHVGAGRVLITSDAQVIREADRVVFPGVGAIRDCMAEIR
RLGFDELVREVSQDRPFLGICVGMQALMERSEENEGVDCIGLFPGQVRFFGKDLHEDGEH
LKVPHMGWNEVKQVVDHPLWHAIPDNGRFYFVHSYYIEAGNPRQVVGRGHYGNDFAAALA
DGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW