Protein Info for Psest_0149 in Pseudomonas stutzeri RCH2

Annotation: nitrate ABC transporter, permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 transmembrane" amino acids 38 to 55 (18 residues), see Phobius details amino acids 102 to 121 (20 residues), see Phobius details amino acids 133 to 153 (21 residues), see Phobius details amino acids 159 to 180 (22 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 224 to 246 (23 residues), see Phobius details amino acids 257 to 279 (23 residues), see Phobius details TIGR01183: nitrate ABC transporter, permease protein" amino acids 77 to 278 (202 residues), 322.8 bits, see alignment E=4.2e-101 PF00528: BPD_transp_1" amino acids 113 to 283 (171 residues), 100.3 bits, see alignment E=5.6e-33

Best Hits

Swiss-Prot: 63% identical to NRTB_PHOLA: Nitrate import permease protein NrtB (nrtB) from Phormidium laminosum

KEGG orthology group: K02050, sulfonate/nitrate/taurine transport system permease protein (inferred from 98% identity to psa:PST_4093)

Predicted SEED Role

"Bicarbonate transport system permease protein" in subsystem CO2 uptake, carboxysome

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFF5 at UniProt or InterPro

Protein Sequence (289 amino acids)

>Psest_0149 nitrate ABC transporter, permease protein (Pseudomonas stutzeri RCH2)
MNANVKLQPASAEPVSIVRPSRLAALARRSGRYAVQQLVPPLVILLVLGVIWEMLCSGAD
AALPPPSQVVAETWELIIDPFYDNGGNDVGLAWQLLASLQRVAVGYLLAVVAGVGLGVLI
GQSDWAMRGLDPIFQVLRTVPPLAWLPLSLAGFQDSHPSALFVIFITAIWPIIINTAVGI
RNIPQDYRNVAQVLQLNGFEYFKTIMLPAAAPYIFTGLRIGVGLSWLAIIAAEMLIGGVG
IGFFIWDAWNASRISDIILALVYIGVVGFVLDRLVAFVGQRITRGIPAN