Protein Info for Psest_1501 in Pseudomonas stutzeri RCH2

Annotation: TolA protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details TIGR02794: protein TolA" amino acids 14 to 328 (315 residues), 179.3 bits, see alignment E=1.3e-56 PF13103: TonB_2" amino acids 248 to 311 (64 residues), 39.5 bits, see alignment E=5e-14 TIGR01352: TonB family C-terminal domain" amino acids 255 to 317 (63 residues), 43.3 bits, see alignment E=3.6e-15 PF03544: TonB_C" amino acids 264 to 305 (42 residues), 28.9 bits, see alignment 1.3e-10

Best Hits

Swiss-Prot: 70% identical to TOLA_PSEAE: Tol-Pal system protein TolA (tolA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03646, colicin import membrane protein (inferred from 90% identity to psa:PST_2804)

Predicted SEED Role

"TolA protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKZ4 at UniProt or InterPro

Protein Sequence (333 amino acids)

>Psest_1501 TolA protein (Pseudomonas stutzeri RCH2)
MQQRDSSPSQSYFWPTVLAVGLHIIIFGMLFVSFSMTPELPPSKPIVQATLYQLKSQSQA
TTQTNQKIAGEAKKTAAKQFESEQMEQRKVEQEKQAAAARAAEQKKAEEARKADAAKAAA
EKAAAAKKAEEAKKVEQQKQAEIAKKKAAEDLAKQKAAEEAKKKAAEEAKRKAAEEAKKK
AAAEAAKKKAAEDAKKKAAADAARKAAEDKKAQALAELLSDTTERQQALADTHGDQVAGN
FDDLIRLRAAEGWTRPPSARNNMTVQLQVNMLPDGTITNVSVSRSSGDVPFDNSAVAAVK
NIGRLTEMQGLSPQDFQPYRSFKMTFTPEDLAL