Protein Info for Psest_1464 in Pseudomonas stutzeri RCH2
Annotation: Selenocysteine lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to CSD_PSEPK: Probable cysteine desulfurase (csdA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K01766, cysteine sulfinate desulfinase [EC: 4.4.1.-] (inferred from 93% identity to psa:PST_2840)MetaCyc: 42% identical to cysteine sulfinate desulfinase (Escherichia coli K-12 substr. MG1655)
2.8.7.M1 [EC: 2.8.7.M1]
Predicted SEED Role
"Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA" in subsystem Alanine biosynthesis (EC 2.8.1.7)
MetaCyc Pathways
- superpathway of L-alanine biosynthesis (4/4 steps found)
- superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
- superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
- L-alanine biosynthesis III (1/1 steps found)
- L-cysteine degradation IV (1/1 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
- molybdopterin biosynthesis (4/6 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
- cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
- bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
- tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
- tRNA-uridine 2-thiolation and selenation (bacteria) (5/11 steps found)
- [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
- tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.8.1.7
Use Curated BLAST to search for 2.8.1.7 or 2.8.7.M1 or 4.4.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GH21 at UniProt or InterPro
Protein Sequence (401 amino acids)
>Psest_1464 Selenocysteine lyase (Pseudomonas stutzeri RCH2) MALFSPWRADFPALAVLEGQGQTYLDSAATAQKPQALIDALSGYYTCGAANVHRAQHQPA ERATRAYEDARIKVAKWLNAACPTEIVFTRGATEALNLLAYGLEHLIEADDEIVISALEH HANLLPWQQLAKRRNARLQVLPIDASGRIDLAAAAALIGPRTRLLAVSQLSNVLGCWQPL DQLMRLAKAQGALTVVDGAQGVVHGRHDMTTLGCDFYVLSSHKLYGPDGVGALYGRGESL LQLRHWQFGGEMVRIADYQSAEFHAAPLGFEAGTPPISGVIGLGATLDYLAGLDLTAVDR HEQALHAQLLAGLSARDGIHLLGSPQLALASFVVEGVHHADLGHLLTEQGIAVRSGNHCA MPLMKHLGLDGATRVSLGLYNDEADLERFFSALDQSLELLQ