Protein Info for PGA1_c14370 in Phaeobacter inhibens DSM 17395

Annotation: DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 PF01653: DNA_ligase_aden" amino acids 17 to 330 (314 residues), 351.7 bits, see alignment E=9.3e-109 TIGR00575: DNA ligase, NAD-dependent" amino acids 24 to 745 (722 residues), 693.4 bits, see alignment E=1.5e-212 PF03120: DNA_ligase_OB" amino acids 334 to 421 (88 residues), 100.2 bits, see alignment E=1.2e-32 PF03119: DNA_ligase_ZBD" amino acids 431 to 457 (27 residues), 41.9 bits, see alignment (E = 1.8e-14) PF12826: HHH_2" amino acids 541 to 572 (32 residues), 28.9 bits, see alignment (E = 2.5e-10) PF00533: BRCT" amino acids 677 to 749 (73 residues), 49.9 bits, see alignment E=7.9e-17

Best Hits

Swiss-Prot: 74% identical to DNLJ_RUEST: DNA ligase (ligA) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 74% identity to sit:TM1040_1398)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EYZ5 at UniProt or InterPro

Protein Sequence (752 amino acids)

>PGA1_c14370 DNA ligase LigA (Phaeobacter inhibens DSM 17395)
MTQSGNNNLKSLTSDEARARFLLLQDELRAADLAYHQADDPEISDADYDAKKREYRALAE
HFPELADGATALETVGAPVASGFGKITHAQRMMSLGNAFDDEDVRDFDKGLRKYLGLGAS
DPLAYTAEPKIDGLSLSLRYENGKLIHAATRGDGAVGENVTENARTIADIPQEIAAAPAV
LEVRGEVYMSHEDFAALNDRHAAQGGKTFANPRNAAAGSLRQLNAEITRSRPLRFFAYSW
GELSEPLAETQFEALTRLREMGFQTNPLTKYCSTTDALIAQYRLIEAQRATLGYDIDGVV
YKVDALALQARLGFRSTTPRWAIAHKFPAELAWTRLEGIDIQVGRTGALSPVARLQPVTV
GGVVVSNATLHNEDYIAGLDSKGEEIRDGKDIRIGDWVQVYRAGDVIPKIADVDLPKRPE
DAVPFAFPAACPECGSDAVREEGDAVRRCTGGLICPAQAVEKLKHFVSRKAFDIDGLGAK
QIEAFYGDAVLTIKTPADIFTLQARDAKTLAKLKNRDGWGETSAANLFAAIEEKRRIELA
RLIFGLGIRHVGEVGARDLALHYADWDAMAAAVDTARPAALAQRAADEAEDRERVAAAQE
NRRARIKEARDRAVATLNVPSEARAAWDDLTGIDGIGATLGVSLSDAFANTEERAAFDAL
RGHLDIIPPEAPQNDSPVTGLTVVFTGTLEKMTRAEAKARAEGLGAKVAGSVSKKTDILV
AGPGAGSKEQKARDLGVRILDEDGWLELIAGA