Protein Info for HP15_1377 in Marinobacter adhaerens HP15

Annotation: dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 1 to 288 (288 residues), 404.1 bits, E=2.1e-125
• PF01180: DHO_dh
  • amino acids 1 to 288 (288 residues), 275.2 bits, E=3.1e-86

Best Hits

Swiss-Prot: 85% identical to PYRD_MARHV: Dihydroorotate dehydrogenase (quinone) (pyrD) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 85% identity to maq:Maqu_1037)

MetaCyc: 56% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.98.1

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

See E4PJF5 at UniProt or InterPro

Protein Sequence (291 amino acids)

>HP15_1377 dihydroorotate dehydrogenase (Marinobacter adhaerens HP15)
MGLDFPNPVGLAAGLDKNADHLDALGALGFGFIEVGTVTPLAQPGNPKPRMFRLPEHQAI
INRMGFNNQGLDHLLARVDQRRYKGVLGINVGKNKDTPNDQSESDYRKGISAVYSRADYI
TVNVSSPNTPGLRDLQFGDSLKGLLEAIKDEQLQCEKEHGRYVPVAVKIAPDMDDDGIRF
VASALRETGLDGVIATNTTISRDAVAGHTHADEAGGLSGAPVREASLRVIRGLYAELGES
LPIIGVGGIMDGESAAEKVLAGAKLVQIYTGFIYRGPELIKEAVEAIRQVK