Protein Info for Psest_1446 in Pseudomonas stutzeri RCH2

Annotation: Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 PF08281: Sigma70_r4_2" amino acids 148 to 195 (48 residues), 34.5 bits, see alignment 2e-12 PF04545: Sigma70_r4" amino acids 150 to 195 (46 residues), 29.2 bits, see alignment 8.2e-11 PF00196: GerE" amino acids 152 to 204 (53 residues), 55.5 bits, see alignment E=5.1e-19

Best Hits

KEGG orthology group: None (inferred from 79% identity to psa:PST_2856)

Predicted SEED Role

"DNA-binding HTH domain-containing protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKV2 at UniProt or InterPro

Protein Sequence (216 amino acids)

>Psest_1446 Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain (Pseudomonas stutzeri RCH2)
MNDHAVSAFQPAQPRVMLLSASRLLHSTLRQHLHDGPDCTLVHMTAPRPEQADAALILLD
VASFDRDECIHLLRQVGDAPVALINAQPDQARRLVETHPWIRGVFYRATPRNIFVRGVQA
MLAGGDWLPRQLMETLLCRYRQLSNSHQVIDELTLREKQILALAGQGLSNAAIGEKLHLS
IHTIKSHVHNALRKLGASNRAQGASLVLAHVGEAVS