Protein Info for Psest_0140 in Pseudomonas stutzeri RCH2

Annotation: choline/carnitine/betaine transport

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 53 to 73 (21 residues), see Phobius details amino acids 94 to 114 (21 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 231 to 253 (23 residues), see Phobius details amino acids 262 to 285 (24 residues), see Phobius details amino acids 316 to 337 (22 residues), see Phobius details amino acids 349 to 368 (20 residues), see Phobius details amino acids 402 to 423 (22 residues), see Phobius details amino acids 449 to 470 (22 residues), see Phobius details amino acids 476 to 497 (22 residues), see Phobius details PF02028: BCCT" amino acids 16 to 497 (482 residues), 619.6 bits, see alignment E=1.7e-190 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 54 to 497 (444 residues), 492.9 bits, see alignment E=4.2e-152

Best Hits

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 82% identity to psa:PST_4102)

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GDD6 at UniProt or InterPro

Protein Sequence (685 amino acids)

>Psest_0140 choline/carnitine/betaine transport (Pseudomonas stutzeri RCH2)
MDAARKLPKAIILFPVFIPAVIVTLLLVIGTISNPELAGEVFSSTLAFITTNFGWFYMLS
VAFFLVFIVGIAMTPWGSIKLGPDHAEPQYSFPAWFAMLFSAGYGIALLFFGVAEPVLHY
ASPPAGAGETVDAAKQAMQIAFFHWGFHIWAIYGLTGLVLAYFSFRHGLPLSMRSALYPI
IGERIHGPIGHVVDIFAILGTLFGIATTLGLSVTQINAGINYLWPSIPISINVQIIAIAI
ITGLAICSVVAGLDKGVKNLSLLNMVLAIGLMLFVFLVGPSIFILETFLQNTGSYLNNII
ERTFNLQAYSRSDWIGNWTLFIFGWTIAWSPFVGLFIAKISRGRTIRQFVFGVMFVPTIF
TFLWFSVFGDTALHMIMVEGYTSLISDVQADNAIALFKLFELLPLTSIASFLAVVLIITF
FVTSSDSGSLVIDSLAAGGALHTPVWQRVFWASIEGIVASTLLLAGGLSALQTMTIASAL
PFAIIMMIAALGMWRALVIEGHHETSLQSHMQGSRLASNAGPGLWKKRLAGMVSFPSREE
VDGFMNTTVLKAMRRVQRELSGQEWAAEVHTDEAHSRLYLEVIKDDQVDFIYEIRAVGYA
MPAFALTEDPETDEQYYRAEVFLRRGGQHYDVYGYDQADIISDILDQFEKYLHFLHISPG
SLPWKMAEHDEMLSDEQDAPKPEAS