Protein Info for HP15_1313 in Marinobacter adhaerens HP15

Annotation: valyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 transmembrane" amino acids 502 to 519 (18 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 930 (930 residues), 1210.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 13 to 629 (617 residues), 517.8 bits, see alignment E=5e-159 PF09334: tRNA-synt_1g" amino acids 37 to 187 (151 residues), 31.2 bits, see alignment E=2.6e-11 PF13603: tRNA-synt_1_2" amino acids 245 to 346 (102 residues), 25.7 bits, see alignment E=1.9e-09 PF08264: Anticodon_1" amino acids 672 to 825 (154 residues), 146.3 bits, see alignment E=1.6e-46 PF10458: Val_tRNA-synt_C" amino acids 885 to 947 (63 residues), 65.3 bits, see alignment 1.2e-21

Best Hits

Swiss-Prot: 67% identical to SYV_COLP3: Valine--tRNA ligase (valS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 68% identity to aha:AHA_3702)

MetaCyc: 67% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PIK2 at UniProt or InterPro

Protein Sequence (950 amino acids)

>HP15_1313 valyl-tRNA synthetase (Marinobacter adhaerens HP15)
MEKTYQPENIERQWYENWESKGYFRPSGEGQSYSIAIPPPNVTGSLHMGHAFQHTIMDTL
TRFKRMQGRNALWTVGTDHAGIATQMVVERKLAAEEDKTRHDLGREEFIKRIWDWKEHSG
GTITRQIRRLGNSVDWDNERFTMDDGFYKAVQEVFIRLYDEGLVYRGKRLVNWDPKLHTA
ISDLEVENKEEKGFFWHLRYPLADGTQTQDGKDYLIVATTRPETMLGDTAVAVHPEDERY
QHLIGKHVMLPLVNRRIPIVADHHADPEKGSGCVKITPAHDFNDYAVGKRNNLPMINVMT
QNANIRDVAEVFNADGTENTELDGTMPGAYAGLTREAARKQIVADMEAEGLVENIEDHVL
SVPRGDRSGLIIEPMLTDQWFADAKTLAKPAIEAVEDGRIQFVPKQYENMYFAWMRDIQD
WCISRQLWWGHRIPAWYDADGNIYVGRSEDEVRQKHNLDANVALEQDEDVLDTWFSSALW
TFGTLGWPEITERLKTFHPTDVLVTGFDIIFFWVARMIMMTMHFMKNEDGTPQVPFHTVY
VTGLIRDEHGDKMSKSKGNVIDPLDMIDGISLDDLLEKRTGNLMQPKLAEKIGKRTKKEF
PEGITAHGTDALRFTLAAMATTGRDINWDMKRLEGYRNFCNKLWNAARYVLMNTEGEDCG
VNDEPVELSLADRWIISELQSCEQDVIRHLDQYRFDLAAYALYEFIWNEYCDWYLELSKP
VLSDEDASAEAKRGTRRTLVRVLESVLRLAHPMMPFITEEIWQRIAPLAGKTGDSIMLQA
YPQPDATKQDSAVTADIEWLKGVIVAVRNIRGEMNISPAKKIPVLLRGKDAEDQRRMNDN
RQFLSSLAKLESLDWFEGDKAPMSATQLVGDMEVLVPMAGLIDKDAELKRLDKELERLQK
EIGRLEGKLGNEKFTAKAPAEVVEKEQEKLRDAQSSQARLSQQRADIEAM