Protein Info for Psest_0134 in Pseudomonas stutzeri RCH2

Annotation: type VI secretion-associated protein, VC_A0119 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 TIGR03362: type VI secretion-associated protein, VC_A0119 family" amino acids 11 to 485 (475 residues), 437 bits, see alignment E=5.2e-135 PF06812: ImpA_N" amino acids 16 to 125 (110 residues), 96.9 bits, see alignment E=9.1e-32 PF16989: T6SS_VasJ" amino acids 224 to 485 (262 residues), 267.3 bits, see alignment E=1.4e-83

Best Hits

KEGG orthology group: K11910, type VI secretion system protein VasJ (inferred from 74% identity to pmy:Pmen_0089)

Predicted SEED Role

"Uncharacterized protein ImpA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GG55 at UniProt or InterPro

Protein Sequence (520 amino acids)

>Psest_0134 type VI secretion-associated protein, VC_A0119 family (Pseudomonas stutzeri RCH2)
MHASALSKHYLELARKPVNDSNFAGDDIRYSSEFELLETELAKAGALHQTTGIDWQKVRE
GSEMLLTSLSKDLRAAAWLTWSLFQRESFAGLQAGLSVLQYLCSNHWAELHPRKPRTRAA
AINWLLPRLEQTFSEDVPVGEQLPLFRGLAEQLHALEQCLSGHLGEDAPLLLPLCRQLDG
MVKRASQGQAQPGTVGAAIAQVKQVASQVFAPGSPIENDKEALKAVRNLQEQARPLTAWW
LKQRTSDLRPLRLSRTLLWLSIDSLPEHNGEQVTALRGIPADKLANYRERFAQGLYADLL
VDLENSTARAPFWLDGQRLAWECLQELAAEQAMREVEIQLALFLQRLPRLEELRFHDGIP
FADAETRAWISGQVMPHVQRAATTRQEEPPAAGSVQPSWELALQEALPLLRKEGLKPAVQ
QLKQGLARARGGRERFFWQLSLARLCYQAKKYELAKTQLEALDQQLEASGLGSWEPDLAL
EVLRLLHSCCELLPQNHAVRESKDEIYRRLCHLDLEVVLD