Protein Info for HP15_1262 in Marinobacter adhaerens HP15

Annotation: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 2 to 197 (196 residues), 45.6 bits, E=5.5e-16
• TIGR01691: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
  • amino acids 2 to 211 (210 residues), 265 bits, E=5e-83
• TIGR01549: HAD hydrolase, family IA, variant 1
  • amino acids 105 to 197 (93 residues), 30.1 bits, E=5.7e-11

Best Hits

Swiss-Prot: 78% identical to MTNC_MARHV: Enolase-phosphatase E1 (mtnC) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K09880, enolase-phosphatase E1 [EC: 3.1.3.77] (inferred from 78% identity to maq:Maqu_0909)

MetaCyc: 51% identical to acireductone synthase [multifunctional] (Klebsiella oxytoca)
Acireductone synthase. [EC: 3.1.3.77, 5.3.2.5]; R83-RXN [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]; 3.1.3.77 [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]

Predicted SEED Role

"2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase (EC 3.1.3.77)" in subsystem Methionine Salvage (EC 3.1.3.77)

MetaCyc Pathways

• L-methionine salvage cycle III (9/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (5/7 steps found)
• L-methionine salvage cycle I (bacteria and plants) (8/12 steps found)
• L-methionine salvage cycle II (plants) (6/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.77 or 3.1.3.87 or 5.3.2.5

Sequence Analysis Tools

See E4PHR1 at UniProt or InterPro

Protein Sequence (230 amino acids)

>HP15_1262 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (Marinobacter adhaerens HP15)
MIRVVLTDIEGTTSSISFVHDVLFPYASEHLPRFVREQHQDSPAVSEQLDAVAEVSGVAR
EDVEGLIQVLETWIREDRKETSLKTLQGMVWEQGYQQGELKGHIYADAADYLQRWHDRGL
RLFVYSSGSVKAQKLIFGFTTEGDFTPFFSGYFDTRIGGKKEAVSYRNILSELGVEACTV
LFLSDVEAELEAAEEAGMQTAWLVREGDLPKTERFVARDFAEVDTLLQKR