Protein Info for Psest_1286 in Pseudomonas stutzeri RCH2

Annotation: inosine-5'-monophosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01302: inosine-5'-monophosphate dehydrogenase
  • amino acids 8 to 456 (449 residues), 633.6 bits, E=9.7e-195
• PF00478: IMPDH
  • amino acids 8 to 475 (468 residues), 554.9 bits, E=1e-170
• PF00571: CBS
  • amino acids 95 to 139 (45 residues), 36.1 bits, 1e-12
  • amino acids 148 to 203 (56 residues), 27.8 bits, 3.9e-10
• PF03060: NMO
  • amino acids 219 to 370 (152 residues), 32.8 bits, E=8e-12

Best Hits

Swiss-Prot: 71% identical to IMDH_ACICA: Inosine-5'-monophosphate dehydrogenase (guaB) from Acinetobacter calcoaceticus

KEGG orthology group: K00088, IMP dehydrogenase [EC: 1.1.1.205] (inferred from 98% identity to psa:PST_3008)

MetaCyc: 65% identical to inosine 5'-monophosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
IMP dehydrogenase. [EC: 1.1.1.205]

Predicted SEED Role

"Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain" in subsystem Purine conversions (EC 1.1.1.205)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• superpathway of purines degradation in plants (11/18 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.205

Sequence Analysis Tools

See L0GKJ8 at UniProt or InterPro

Protein Sequence (489 amino acids)

>Psest_1286 inosine-5'-monophosphate dehydrogenase (Pseudomonas stutzeri RCH2)
MLRISQEALTFDDVLLIPGYSEVLPKDVSLKTRLTREIELNIPLVSAAMDTVTEARLAIA
MAQEGGIGIIHKNMSIEQQAAEVRKVKRHETAIVHDPVTVTPETKISELLRKAHELGFSG
FPVVSGKELVGIVTGRDLRFTPNAGDSVAAIMTPKDKLVTVLEGTGLEEIKTELYKHRIE
KMLVVDGNFHLRGLVTFRDIEKAKTYPLASKDSQGRLRVGAAVGTGADTGERVEALAAAG
VDVVVVDTAHGHSRGVIDRVRWVKENFPQVQVIGGNIATAEAALDLVKAGADAVKVGIGP
GSICTTRIVAGVGVPQISAIANVATALEGTGVPMIADGGIRFSGDLSKAIVAGANAVMMG
SMFAGTEEAPGEIELFQGRSYKAYRGMGSLGAMSQAQGSSDRYFQDSAAGAEKLVPEGIE
GRVPYKGSLAAIIHQLMGGLRASMGYTGSATVDEMRSKPQFVRITGAGMAESHVHDVQIT
KEAPNYRVG