Protein Info for Psest_1255 in Pseudomonas stutzeri RCH2

Annotation: Fe-S protein assembly chaperone HscA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 TIGR01991: Fe-S protein assembly chaperone HscA" amino acids 21 to 616 (596 residues), 907.6 bits, see alignment E=1.8e-277 PF00012: HSP70" amino acids 21 to 600 (580 residues), 611.2 bits, see alignment E=1.8e-187 PF06723: MreB_Mbl" amino acids 126 to 377 (252 residues), 41.8 bits, see alignment E=6.2e-15

Best Hits

Swiss-Prot: 94% identical to HSCA_PSEU5: Chaperone protein HscA homolog (hscA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K04044, molecular chaperone HscA (inferred from 94% identity to psa:PST_3038)

MetaCyc: 65% identical to iron-sulfur cluster biosynthesis chaperone HscA (Escherichia coli K-12 substr. MG1655)
3.6.4.-

Predicted SEED Role

"Chaperone protein HscA" in subsystem Protein chaperones

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGD9 at UniProt or InterPro

Protein Sequence (620 amino acids)

>Psest_1255 Fe-S protein assembly chaperone HscA (Pseudomonas stutzeri RCH2)
MALLQIAEPGQSPQPHQRRLAVGIDLGTTNSLVAALRSGVTAPLADADGQVILPSVVRYH
ADHVEVGAQAKLAAATDPFNTISSVKRLMGRGLADVKQMGEQLPYRFRQAESQMPFIETV
QGAKSPVEVSAEILRALRLRAEESLGGELVGAVITVPAYFDDAQRQATKDAARLAGLNVL
RLLNEPTAAAVAYGLDRQAEGVVAIYDLGGGTFDISILRLTKGVFEVLATGGDTALGGDD
FDHTVADWILECAGVSGDLEPGAQRELLKIACDAKERLTDVDVVTVAYAGWSGELHRETF
DALIEPMIARSLKSCRRAVRDSGVELEEITAVVMVGGSTRVPKVRSAVGQLFGREPLTDI
DPDEVVAIGAAIQAETLAGNNRDGEELLLLDVIPLSLGLETMGGLMEKIIPRNTTIPVAR
AQDFTTYKDGQSAMMIHVLQGERELISDCRSLARFELRGIPPMVAGAAKIRVTFQVDADG
LLSVSARELASGVEASIQVKPSYGLTDGEIARMLEDSFRKADEDRDARALREQLVDAQRL
LEAVEAALAADGERLLSAEERLAIDSQMAGLRGLLDSQDAVAIERQTKRLSQITDAFAAR
RLDSTVKAALAGRRLNDIED